Western blot abreview for Anti-Caspase 12 antibody

Good
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Dorsal root ganglion)
Loading amount
15 µg
Specification
Dorsal root ganglion
Gel Running Conditions
Reduced Denaturing (gel 10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/2000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS 0.1% Tween20

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit HRP-linked secondary antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 42, 38 kDa

Additional data

Additional Notes
The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat
Abcam response
Many thanks for your feedback on ab62484 (Caspase 12 antibody) in WB on rat dorsal root ganglion lysate.

Dr. Karine Thibault

Verified customer

Submitted Oct 13 2010

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