Western blot abreview for Anti-Caspase-12 antibody

Below Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MOLT4)
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Loading amount
100000 cells
Treatment
Bortezomib 0-20hours
Specification
MOLT4
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: CST Anti-rabbit IgG, HRP-linked Antibody #7074
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 38 kDa Non-specific: 70, 90 kDa
Positive control
MOLT4 samples

Additional data

Additional Notes
While main band for cleaved Caspase-12 proform is clearly visible at 38kda, there are several non-specific bands.
Abcam response
Customer has been contacted with troubleshooting advise. The results can be improved by applying variations in antibody dilution factor and blocking buffer and incubation timing.

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Verified customer

Submitted Jun 19 2015

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