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Human Cell lysate - whole cell (MOLT4)
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Non-Abcam antibody was used: CST Anti-rabbit IgG, HRP-linked Antibody #7074
Host species: Goat
Conjugation: HRP polymer
Specific: 38 kDa Non-specific: 70, 90 kDa
While main band for cleaved Caspase-12 proform is clearly visible at 38kda, there are several non-specific bands.
Customer has been contacted with troubleshooting advise. The results can be improved by applying variations in antibody dilution factor and blocking buffer and incubation timing.
Abcam user community
Submitted Jun 19 2015