Western blot abreview for Anti-Caspase-12 antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2 cell line)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine gel)
Loading amount
50000 cells
Specification
HEPG2 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody CS#7074
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Clarity Western ECL (biorad)
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 38, 45 kDa Non-specific: 30, 110 kDa

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Submitted Apr 25 2017

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