Caspase-12 Assay Kit (Fluorometric) (ab65664)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
Overview
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Product name
Caspase-12 Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Assay time
2h 00m -
Product overview
Abcam's Caspase-12 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
Visit our FAQs page for tips and troubleshooting. -
Notes
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2X Reaction Buffer 4 x 2ml ATAD-AFC Substrate 1 x 500µl Cell Lysis Buffer 1 x 100ml DTT 1 x 400µl -
Research areas
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Relevance
Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. Caspase 12 is most highly related to members of the ICE subfamily. However, human caspase 12 has no protease activity. It may reduce cytokine release in response to bacterial lipopolysaccharide during infections and reduces activation of NF-kappa-B in response to TNF. -
Alternative names
- CASP12
- CASP12P1
- Caspase 12
- Inactive caspase 12
Associated products
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Related Products
References (6)
ab65664 has been referenced in 6 publications.
- Kamarehei M et al. Increased expression of endoplasmic reticulum stress-related caspase-12 and CHOP in the hippocampus of EAE mice. Brain Res Bull 147:174-182 (2019). PubMed: 30738137
- Xing J et al. Melatonin ameliorates endoplasmic reticulum stress in N2a neuroblastoma cell hypoxia-reoxygenation injury by activating the AMPK-Pak2 pathway. Cell Stress Chaperones 24:621-633 (2019). PubMed: 30976981
- Hou S et al. Mitofusin-2 regulates inflammation-mediated mouse neuroblastoma N2a cells dysfunction and endoplasmic reticulum stress via the Yap-Hippo pathway. J Physiol Sci 69:697-709 (2019). PubMed: 31134519
- Asano M et al. Normothermic Microwave Irradiation Induces Death of HL-60 Cells through Heat-Independent Apoptosis. Sci Rep 7:11406 (2017). PubMed: 28900243
- Bulatovic I et al. Sublethal caspase activation promotes generation of cardiomyocytes from embryonic stem cells. PLoS One 10:e0120176 (2015). Functional Studies . PubMed: 25763592
- Mitra A et al. Cardioprotective role of P38 MAPK during myocardial infarction via parallel activation of a-crystallin B and Nrf2. J Cell Physiol 229:1272-82 (2014). Functional Studies . PubMed: 24464634
