Caspase 12 Assay Kit (Fluorometric) (ab65664)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameCaspase 12 Assay Kit (Fluorometric)
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Sample typeTissue Extracts, Cell Lysate
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Assay typeEnzyme activity
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Assay time2h 00m
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Product overview
Abcam's Caspase 12 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 12 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
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Notes
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 100 tests 2X Reaction Buffer 4 x 2ml ATAD-AFC Substrate 1 x 500µl Cell Lysis Buffer 1 x 100ml DTT 1 x 400µl -
Research areas
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RelevanceCaspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. Caspase 12 is most highly related to members of the ICE subfamily. However, human caspase 12 has no protease activity. It may reduce cytokine release in response to bacterial lipopolysaccharide during infections and reduces activation of NF-kappa-B in response to TNF.
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Alternative names
- CASP12
- CASP12P1
- Caspase 12
- Inactive caspase 12
Associated products
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Related Products
Protocols
References
This product has been referenced in:
- Asano M et al. Normothermic Microwave Irradiation Induces Death of HL-60 Cells through Heat-Independent Apoptosis. Sci Rep 7:11406 (2017). Read more (PubMed: 28900243) »
- Bulatovic I et al. Sublethal caspase activation promotes generation of cardiomyocytes from embryonic stem cells. PLoS One 10:e0120176 (2015). Functional Studies . Read more (PubMed: 25763592) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"