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Abcam's Caspase 12 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 12 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
|2X Reaction Buffer||4 x 2ml|
|ATAD-AFC Substrate||1 x 500µl|
|Cell Lysis Buffer||1 x 100ml|
|DTT||1 x 400µl|
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