Recombinant HRP Anti-Caspase-14 antibody [EPR12927] (ab200079)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12927] to Caspase-14
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Caspase-14 antibody [EPR12927]
See all Caspase-14 primary antibodies -
Description
HRP Rabbit monoclonal [EPR12927] to Caspase-14 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skin lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12927 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
Target
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Function
Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. -
Tissue specificity
Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels. -
Sequence similarities
Belongs to the peptidase C14A family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23581 Human
- Entrez Gene: 12365 Mouse
- Entrez Gene: 299587 Rat
- Omim: 605848 Human
- SwissProt: P31944 Human
- SwissProt: O89094 Mouse
- Unigene: 466057 Human
- Unigene: 20940 Mouse
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Alternative names
- Apoptosis related cysteine protease antibody
- CASP 14 antibody
- CASP-14 antibody
see all
Images
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HRP Anti-Caspase-14 antibody [EPR12927] (ab200079) at 1/5000 dilution + Skin (Human) Tissue Lysate - adult normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200079 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab200079 has not yet been referenced specifically in any publications.