Overview

  • Product name

    Caspase 2 (active) FITC Staining Kit
    See all Caspase-2 kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Enzyme activity
  • Assay time

    2h 00m
  • Product overview

    Abcam's Caspase 2 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 2 in living cells. The assay utilizes the caspase 2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 2 in apoptotic cells. The FITC label allows detection of activated caspase 2 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of caspases plays a central role in apoptosis.

    Other caspase and apoptosis assays

    Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

Protocols

References

ab65612 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the cells in suspension. However, I have not seen a reference or data for this, so I do not know if the trypsinization will itself affect the caspase cascade.

If you go with what we discussed, treating and staining the cells while adhered, you should consider the possibility that some of the apoptotic cells may detach from the culture plate or flask as a consequence of the apoptosis-inducing treatment. (I am assuming you are comparing cells +/- treatment). If this is the case, treating and staining trypsinized cells might be a better approach, so that none of the cells are lost (that is, the detached, dying cells) during the assay.

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