Recombinant Anti-Caspase-2 antibody [EPR16796] (ab179520)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-Caspase-2 antibody [EPR16796]
See all Caspase-2 primary antibodies -
Description
Rabbit monoclonal [EPR16796] to Caspase-2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Mouse Caspase-2 aa 150-350. The exact sequence is proprietary.
Database link: P29594 -
Positive control
- WB: HeLa whole cell lysates treated with 4uM camptothecin for 4 hours; C6 whole cell lysates treated with 1uM staurosporine for 4 hours; RAW 264.7 whole cell lysate; Human fetal kidney lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16796 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab179520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 48, 33 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. -
Tissue specificity
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe mature protease can process its own propeptide, but not that of other caspases. - Information by UniProt
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Database links
- Entrez Gene: 835 Human
- Entrez Gene: 12366 Mouse
- Entrez Gene: 64314 Rat
- Omim: 600639 Human
- SwissProt: P42575 Human
- SwissProt: P29594 Mouse
- SwissProt: P55215 Rat
- Unigene: 368982 Human
see all -
Alternative names
- CASP 2 antibody
- CASP-2 antibody
- Casp2 antibody
see all
Images
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![Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)](https://www.abcam.com/ps/products/179/ab179520/Images/ab179520-253293-anti-caspase-2-antibody-epr16796-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase-2 knockout HAP1 cell lysate (20 µg)Lanes 1 and 2: Merged signal (red and green). Green - ab17952 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab179520 was shown to recognize Caspase-2 when Caspase-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-2 knockout samples were subjected to SDS-PAGE. ab179520 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)](https://www.abcam.com/ps/products/179/ab179520/Images/ab179520-240422-anti-caspase-2-antibody-epr16796-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)All lanes : Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 4uM camptothecin for 4 hours
Lane 2 : Untreated HeLa whole cell lysate
Lane 3 : C6 (Rat glial tumor cells) whole cell lysate treated with 1uM staurosporine for 4 hours
Lane 4 : Untreated C6 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 33,48 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe 33KDa band (cleaved caspase 2) contains subunit p18.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)](https://www.abcam.com/ps/products/179/ab179520/Images/ab179520-240421-anti-caspase-2-antibody-epr16796-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/1000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 33,48 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe 33KDa band (cleaved caspase 2) contains subunit p18.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)](https://www.abcam.com/ps/products/179/ab179520/Images/ab179520-240420-anti-caspase-2-antibody-epr16796-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3 minutesThe 33KDa band (cleaved caspase 2) contains subunit p18.
Blocking/Dilution buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zierhut C et al. The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death. Cell 178:302-315.e23 (2019). Read more (PubMed: 31299200) »
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). Read more (PubMed: 30377371) »
