Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Caspase-2 antibody [EPR16796] (ab179520)

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Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
  • Anti-Caspase-2 antibody [EPR16796] (ab179520)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16796] to Caspase-2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caspase-2 antibody [EPR16796]
    See all Caspase-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16796] to Caspase-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysates treated with 4uM camptothecin for 4 hours; C6 whole cell lysates treated with 1uM staurosporine for 4 hours; RAW 264.7 whole cell lysate; Human fetal kidney lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 48, 33 kDa (predicted molecular weight: 51 kDa).
Notes
WB
1/1000. Detects a band of approximately 48, 33 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.

  • Tissue specificity

    Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.

  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.

  • Post-translational
    modifications

    The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CASP 2 antibody
    • CASP-2 antibody
    • Casp2 antibody
    • CASP2_HUMAN antibody
    • Caspase 2 antibody
    • Caspase 2 apoptosis related cysteine peptidase antibody
    • Caspase-2 subunit p12 antibody
    • Caspase2 antibody
    • ICH 1 antibody
    • ICH 1 protease antibody
    • ICH 1L antibody
    • ICH1 antibody
    • ICH1 protease antibody
    • ICH1L antibody
    • NEDD-2 antibody
    • NEDD2 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 2 antibody
    • PPP1R57 antibody
    • Protease ICH-1 antibody
    • Protein phosphatase 1 regulatory subunit 57 antibody
    see all

Images

  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
    Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Caspase-2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab17952 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab179520 was shown to recognize Caspase-2 when Caspase-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-2 knockout samples were subjected to SDS-PAGE. ab179520 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
    Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
    All lanes : Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 4uM camptothecin for 4 hours
    Lane 2 : Untreated HeLa whole cell lysate
    Lane 3 : C6 (Rat glial tumor cells) whole cell lysate treated with 1uM staurosporine for 4 hours
    Lane 4 : Untreated C6 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 33,48 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The 33KDa band (cleaved caspase 2) contains subunit p18.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
    Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
    Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/1000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 33,48 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The 33KDa band (cleaved caspase 2) contains subunit p18.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
    Western blot - Anti-Caspase-2 antibody [EPR16796] (ab179520)
    Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    The 33KDa band (cleaved caspase 2) contains subunit p18.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Caspase-2 antibody [EPR16796] (ab179520)
    Anti-Caspase-2 antibody [EPR16796] (ab179520)

References (5)

Publishing research using ab179520? Please let us know so that we can cite the reference in this datasheet.

ab179520 has been referenced in 5 publications.

  • Mollica G  et al. L-carnitine supplementation attenuates NAFLD progression and cardiac dysfunction in a mouse model fed with methionine and choline-deficient diet. Dig Liver Dis 52:314-323 (2020). PubMed: 31607566
  • Zierhut C  et al. The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death. Cell 178:302-315.e23 (2019). PubMed: 31299200
  • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
  • Liu J  et al. BECN1-dependent CASP2 incomplete autophagy induction by binding to rabies virus phosphoprotein. Autophagy 13:739-753 (2017). PubMed: 28129024
  • Yao W  et al. Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol-induced heart failure by downregulation of tumor necrosis factor-a and nuclear factor-?B. Exp Ther Med 14:4104-4110 (2017). PubMed: 29104628

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