Recombinant Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
![Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)](https://www.abcam.com/ps/products/247/ab247211/Images/ab247211-403201-anti-caspase-2-antibody-y154-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y154] to Caspase-2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Caspase-2 antibody [Y154] - BSA and Azide free
See all Caspase-2 primary antibodies -
Description
Rabbit monoclonal [Y154] to Caspase-2 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody is specific for the pro form and the p18 cleaved form of Caspase-2.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Caspase-2 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P42575 -
General notes
ab247211 is the carrier-free version of ab32021 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247211 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Y154 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa). Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form).
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Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. -
Tissue specificity
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe mature protease can process its own propeptide, but not that of other caspases. - Information by UniProt
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Database links
- Entrez Gene: 835 Human
- Omim: 600639 Human
- SwissProt: P42575 Human
- Unigene: 368982 Human
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Alternative names
- CASP 2 antibody
- CASP-2 antibody
- Casp2 antibody
see all
Images
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Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
This data was developed using ab32021, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)](https://www.abcam.com/ps/products/247/ab247211/Images/ab247211-399255-anti-caspase-2-antibody-y154-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution + Jurkat cell lysate
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa (possible cleavage fragment)This data was developed using ab32021, the same antibody clone in a different buffer formulation.
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![Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)](https://www.abcam.com/ps/products/247/ab247211/Images/ab247211-3-benefits-of-recombinant-antibodies.png)
Anti-Caspase-2 antibody [Y154] - BSA and Azide free (ab247211)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247211 has not yet been referenced specifically in any publications.
