Product nameCaspase 2 Assay Kit (Colorimetric)
See all Caspase 2 kits
Sample typeTissue Extracts, Cell Lysate
Assay typeEnzyme activity
Assay time2h 00m
Abcam's Caspase 2 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate VDVAD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests 1 kit 2X Reaction Buffer 4 x 2ml 4 x 2ml Cell Lysis Buffer 1 x 100ml 1 x 100ml Dilution Buffer 1 x 100ml 1 x 100ml DTT 1 x 400µl 1 x 400µl VDVAD-pNA 1 x 500µl 1 x 500µl
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
Tissue specificityExpressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe mature protease can process its own propeptide, but not that of other caspases.
- Information by UniProt
ab39830 has not yet been referenced specifically in any publications.