Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
Product nameCaspase-2 Assay Kit (Fluorometric)
See all Caspase-2 kits
Sample typeTissue Extracts, Cell Lysate
Assay typeEnzyme activity
Assay time2h 00m
Abcam's Caspase-2 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml DTT 1 x 400µl VDVAD-AFC 1 x 500µl
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
Tissue specificityExpressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe mature protease can process its own propeptide, but not that of other caspases.
- Information by UniProt
- Casp 2L Pro
ab39794 has not yet been referenced specifically in any publications.