Product nameCaspase 2 Inhibitor Assay Kit
See all Caspase 2 kits
Sample typeAdherent cells, Suspension cells
Assay time1h 30m
Abcam's Caspase 2 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VDVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 2 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 2 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-2 1 x 100 units Caspase Inhibitor, Z-VAD-FMK 1 x 10µl Caspase Substrate VDVAD-AFC 1 x 0.5ml DTT 1 x 100µl
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
Tissue specificityExpressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe mature protease can process its own propeptide, but not that of other caspases.
- Information by UniProt
Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using VDVAD-AFC as caspase 2 substrate and following Caspase 2 Inhibitor Drug Detection Kit (ab102490) protocol. Lanes:
1.- Background Control: no Caspase 2, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 2, +z-VAD-FMK
3.- Positive Control: + Caspase 2, no z-VAD-FMK
4.- Caspase 2 + 2.5µM z-VAD-FMK
5.- Caspase 2 + 10µM z-VAD-FMK
6.- Caspase 2 + 40µM z-VAD-FMK
ab102490 has not yet been referenced specifically in any publications.