Anti-Caspase-2L antibody (ab18737)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-Caspase-2L antibody
See all Caspase-2L primary antibodies -
DescriptionRabbit polyclonal to Caspase-2L
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Host speciesRabbit
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Specificityab18737 detects the full length and small subunit of caspase-2. In human samples it also detects a 30kDa intermediate fragment.
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
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Positive control
- Mouse small intestine lysate. This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: MCF-7.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab18737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).Can be blocked with Mouse Caspase-2L peptide (ab38266). Full length protein and a smaller band of about 12 kDa (small subunit). |
Target
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RelevanceFunction: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Tissue specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. PTM: The mature protease can process its own propeptide, but not that of other caspases.
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Database links
- SwissProt: P42575-1 Human
- SwissProt: P29594 Mouse
- SwissProt: P55215 Rat
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FormSpecific for P42575-1; the isoform that has been chosen as the 'canonical' sequence.
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Alternative names
- CASP2 antibody
- ICH-1L antibody
- ICH1 antibody
see all
Images
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Western blot - Anti-Caspase-2L antibody (ab18737)WB of Caspase-2 detected with ab18737. Lanes 1 and 2: untreated cell lysate Lanes 3 and 4: treated cell lysate
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Immunocytochemistry/ Immunofluorescence - Anti-Caspase-2L antibody (ab18737)ICC/IF image of ab18737 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab18737 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-rabbit DyLight® 488 (ab96899) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Sandow JJ et al. ER stress does not cause upregulation and activation of caspase-2 to initiate apoptosis. Cell Death Differ N/A:N/A (2013). Mouse . Read more (PubMed: 24292555) »
- Jelínek M et al. Caspase-2 is involved in cell death induction by taxanes in breast cancer cells. Cancer Cell Int 13:42 (2013). WB . Read more (PubMed: 23672670) »
