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    caspase-2l-antibody-ab18737.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Anti-Caspase-2L antibody (ab18737)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (3)

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Western blot - Anti-Caspase-2L antibody (ab18737)

    Key features and details

    • Rabbit polyclonal to Caspase-2L
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Caspase-2L antibody
      See all Caspase-2L primary antibodies
    • Description

      Rabbit polyclonal to Caspase-2L
    • Host species

      Rabbit
    • Specificity

      ab18737 detects the full length and small subunit of caspase-2. In human samples it also detects a 30kDa intermediate fragment.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to Mouse Caspase-2L. Immuogen: Synthetic peptide surrounding amino acid 417 of mouse caspase-2
      Database link: P42575
      (Peptide available as ab38266)

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: PBS, 30% Glycerol, 0.5% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Caspases etc
      • Caspases
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Caspases
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Other proteases
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Caspases
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Mouse small intestine tissue lysate - total protein (ab7939)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab18737 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa). Full length protein and a smaller band of about 12 kDa (small subunit).
    Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa). Full length protein and a smaller band of about 12 kDa (small subunit).

    Target

    • Relevance

      Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Tissue specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. PTM: The mature protease can process its own propeptide, but not that of other caspases.
    • Database links

      • SwissProt: P42575-1 Human
      • Form

        Specific for P42575-1; the isoform that has been chosen as the 'canonical' sequence.
      • Alternative names

        • CASP2 antibody
        • ICH-1L antibody
        • ICH1 antibody
        • NEDD-2 antibody
        • NEDD2 antibody
        • Protease ICH-1 antibody
        see all

      Images

      • Western blot - Anti-Caspase-2L antibody (ab18737)
        Western blot - Anti-Caspase-2L antibody (ab18737)

        WB of Caspase-2 detected with ab18737. Lanes 1 and 2: untreated cell lysate Lanes 3 and 4: treated cell lysate

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (3)

      Publishing research using ab18737? Please let us know so that we can cite the reference in this datasheet.

      ab18737 has been referenced in 3 publications.

      • Sandow JJ  et al. ER stress does not cause upregulation and activation of caspase-2 to initiate apoptosis. Cell Death Differ N/A:N/A (2013). Mouse . PubMed: 24292555
      • Jelínek M  et al. Caspase-2 is involved in cell death induction by taxanes in breast cancer cells. Cancer Cell Int 13:42 (2013). WB . PubMed: 23672670
      • Zhang Y  et al. Huntingtin inhibits caspase-3 activation. EMBO J 25:5896-906 (2006). PubMed: 17124493

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-Caspase 2 antibody

      Poor
      Abreviews
      Abreviews
      Application
      Western blot
      Loading amount
      50 µg
      Gel Running Conditions
      Reduced Denaturing (12% gel, MOPS buffer)
      Sample
      Mouse Cell lysate - whole cell (C17.2 cell line)
      Specification
      C17.2 cell line
      Treatment
      Heat-shock for 2 hours at 43 degrees
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 18 2013

      Question

      1. Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001

      2. Alexa Fluor® 488 goat anti-rabbit IgG Cat # A- 11008

      3. Cy3® goat anti-mouse IgG (H+L) *2mg/mL Cat A 10521

      4. Cy3® goat anti-rabbit IgG (H+L) *2mg/mL A10520

      5. Cdc2 p34 200 ìg (PSTAIRE sc-53(Epitope is of human origin

      6. Nanog Antibody (A-11) sc-374001

      7. Oct-3/4 Antibody (H-134) sc-9081

      8. Anti Sox2 Cat. No.sc-17320

      9. caspase-3 Antibody (H- 277): sc-7148

      10. caspase-2L Antibody (C- 19): sc-626

      11. Blasticidin S HCl R210-01 50mg)



      Please let us have best alternate products for the above at your earliest.


      Sincere regards,

      Read More

      Abcam community

      Verified customer

      Asked on Sep 24 2012

      Answer

      Thank you for contacting us.

      1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

      or ab96879; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-pre-adsorbed-ab96879.html

      2. ab96895; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96895.html

      3. ab97035; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-adsorbed-ab97035.html

      4. ab97075; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Cy3-pre-adsorbed-ab97075.html

      5. ab10345; https://www.abcam.com/PSTAIR-antibody-PSTAIR-ab10345.html

      6. We do not have anti Nanog mouse monoclonal that customer is after. We have similar rabbit polyclonal antibodies available. The links are

      https://www.abcam.com/Nanog-antibody-ab70482.html

      https://www.abcam.com/Nanog-antibody-ab90640.html

      7. ab109187; https://www.abcam.com/Oct4-antibody-EPR2053-2-ab109187.html

      8. ab97959; https://www.abcam.com/SOX2-antibody-ab97959.html

      9. ab131000; https://www.abcam.com/Caspase-3-antibody-ab131000.html

      10. ab18737; https://www.abcam.com/Caspase-2-antibody-ab18737.html

      11. We do not sell this chemical.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Sep 24 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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