Overview

  • Product name
    Anti-Caspase-2L antibody
    See all Caspase-2L primary antibodies
  • Description
    Rabbit polyclonal to Caspase-2L
  • Host species
    Rabbit
  • Specificity
    ab18737 detects the full length and small subunit of caspase-2. In human samples it also detects a 30kDa intermediate fragment.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-2L. Immuogen: Synthetic peptide surrounding amino acid 417 of mouse caspase-2
    Database link: P42575
    (Peptide available as ab38266)

  • Positive control
    • Mouse small intestine lysate. This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

Applications

Our Abpromise guarantee covers the use of ab18737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).Can be blocked with Mouse Caspase-2L peptide (ab38266). Full length protein and a smaller band of about 12 kDa (small subunit).

Target

  • Relevance
    Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Tissue specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. PTM: The mature protease can process its own propeptide, but not that of other caspases.
  • Database links
    • Form
      Specific for P42575-1; the isoform that has been chosen as the 'canonical' sequence.
    • Alternative names
      • CASP2 antibody
      • ICH-1L antibody
      • ICH1 antibody
      • NEDD-2 antibody
      • NEDD2 antibody
      • Protease ICH-1 antibody
      see all

    Images

    • Western blot - Anti-Caspase-2L antibody (ab18737)
      Western blot - Anti-Caspase-2L antibody (ab18737)

      WB of Caspase-2 detected with ab18737. Lanes 1 and 2: untreated cell lysate Lanes 3 and 4: treated cell lysate

    • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-2L antibody (ab18737)
      Immunocytochemistry/ Immunofluorescence - Anti-Caspase-2L antibody (ab18737)

      ICC/IF image of ab18737 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab18737 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-rabbit DyLight® 488 (ab96899) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    This product has been referenced in:
    • Sandow JJ  et al. ER stress does not cause upregulation and activation of caspase-2 to initiate apoptosis. Cell Death Differ N/A:N/A (2013). Mouse . Read more (PubMed: 24292555) »
    • Jelínek M  et al. Caspase-2 is involved in cell death induction by taxanes in breast cancer cells. Cancer Cell Int 13:42 (2013). WB . Read more (PubMed: 23672670) »

    See all 3 Publications for this product

    Publishing research using ab18737? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    2 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-Caspase 2 antibody

     Poor
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12% gel, MOPS buffer)
    Sample
    Mouse Cell lysate - whole cell (C17.2 cell line)
    Specification
    C17.2 cell line
    Treatment
    Heat-shock for 2 hours at 43 degrees
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Oct 18 2013

    1. Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001

    2. Alexa Fluor® 488 goat anti-rabbit IgG Cat # A- 11008

    3. Cy3® goat anti-mouse IgG (H+L) *2mg/mL Cat A 10521

    4. Cy3® goat anti-rabbit IgG (H+L) *2mg/mL A...

    Read More

    Thank you for contacting us.

    1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

    or ab96879; http...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up