Product nameCaspase 3 (active) FITC Staining Kit
See all Caspase 3 kits
Sample typeAdherent cells, Suspension cells
Assay typeEnzyme activity
Assay time2h 00m
Abcam's Caspase 3 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 3 in living cells. The assay utilizes the caspase 3 inhibitor, DEVD-FMK, conjugated to FITC (FITC-DEVD-FMK) as a marker. FITC-DEVD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 3 in apoptotic cells. The FITC label allows detection of activated caspase-3 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
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Activation of caspases plays a central role in apoptosis.
Tested applicationsSuitable for: FM, Flow Cytmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests FITC-DEVD-FMK 1 x 100µl Wash Buffer 2 x 100ml Z-VAD-FMK 1 x 10µl
RelevanceThe caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as an inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (e.g. mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Our Abpromise guarantee covers the use of ab65613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
This product has been referenced in:
- Martínez-Torres AC et al. IMMUNEPOTENT CRP induces cell cycle arrest and caspase-independent regulated cell death in HeLa cells through reactive oxygen species production. BMC Cancer 18:13 (2018). Flow Cyt ; Human . Read more (PubMed: 29298674) »
- Våtsveen TK et al. Artesunate shows potent anti-tumor activity in B-cell lymphoma. J Hematol Oncol 11:23 (2018). Read more (PubMed: 29458389) »