• Product name
  • Description
    Rabbit polyclonal to Caspase-3
  • Host species
  • Specificity
    Ab44976 does not recognize the p12 or p17 activated forms of caspase-3.
  • Tested applications
    Suitable for: IP, IHC-P, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Caspase-3.
    Database link: P42574

  • Positive control
    • Jurkat cells. Tonsil.



Our Abpromise guarantee covers the use of ab44976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/2000.
IHC-P 1/1000.
WB 1/500. Predicted molecular weight: 32 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 20664058
IHC-Fr Use at an assay dependent concentration. See Abreviews


  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificity
    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similarities
    Belongs to the peptidase C14A family.
  • Post-translational
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all


  • ab44976, at 1/100, staining Caspase-3 in mouse brain tissue sections by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% BSA for 1 hour at 25°C and then incubated with ab44976, for 12 hours at 4°C. A donkey polyclonal to rabbit Ig, diluted 1/500 was used as the secondary antibody. The green shows staining of caspase 3 and the blue DAPI.

    See Abreview

  • Western blot analysis of caspase-3 precursor expression in HuT 78 (A) and CCRF-HSB-2 (B) whole cell lysates.


This product has been referenced in:
  • Xie X  et al. Homeobox C10 knockdown suppresses cell proliferation and promotes cell apoptosis in osteosarcoma cells through regulating caspase 3. Onco Targets Ther 11:473-482 (2018). Read more (PubMed: 29403292) »
  • Zhao Q  et al. Rhein protects against cerebral ischemic-/reperfusion-induced oxidative stress and apoptosis in rats. Int J Mol Med 41:2802-2812 (2018). Read more (PubMed: 29436613) »

See all 37 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed on the datasheets. Therefore, any Caspase 3 antibody tes...

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