Question (68618) | Caspase-3 Assay Kit (Colorimetric) (ab39401)

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Question

Dear Technical Team,

Hope this e-mail finds you well!

Since, it is not mentioned in the protocol of Caspase 3 Assay Kit (Colorimetric) (ab39401). Kindly suggest us on how to proceed with tissues or how to make single cell suspension as desired by the customer.

Looking forward towards your valued response on it.

With Best Regards,

Answer

Thank you for contacting us and your interest in our products.

The Caspase 3 Assay Kit (Colorimetric) (ab39401) can be used with tissue samples and we would suggest following the following protocol:

1. Induce apoptosis in tissue (10-100) mg by desired method. Concurrently have control without induction.

2. Re-suspend tissue in 50-400 μl of chilled Cell Lysis Buffer and homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed, by viewing the cells under the microscope.

3. Centrifuge for 1 min in a microcentrifuge (10,000 x g).

Subsequently follow the remaining protocol as described with cell samples.
I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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