Question (73529) | Caspase-3 Assay Kit (Colorimetric) (ab39401)

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Question

I want to assay caspase 3 activity in platelet rich plasma (PRP) during storage. Do i use abcam caspase 3 colorimetric assay kit for this purpose? Lysis buffer in the kit is suitable for platelet lysis ? If you have any protocol to assay platelet caspase 3 activity by this kit, please send me, beacuse i want to use this kit for my project.  

Answer

This kit is good just for cell and tissue lysates but not plasma. If the you are able to collect the platelets from the plasma in a clean manner you will be able to use the kit with the recommended protocol.

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