For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I am interested in using Abcam Caspase 3 assay kit (ab39383) in samples from rat's heart. However, I already have the tissue lysate. Do the protease inhibitor that I added will interfere with the assay?
Asked on May 31 2013
I am afraid that your protease inhibitors might interefere with the assay. Since Caspase is a cysteine protease it is probably inhibited by most protease inhibitor cocktails. The assay is based on the catalytic clevage of the substrate DEVD-AFC by Caspase-3. Inhibition of caspase would thus interfere with the measurement.
Answered on May 31 2013