Overview

  • Product name

    Anti-Caspase-3 (phospho S150) antibody
    See all Caspase-3 primary antibodies
  • Description

    Rabbit polyclonal to Caspase-3 (phospho S150)
  • Host species

    Rabbit
  • Specificity

    Caspase-3 (Phospho-Ser150) antibody detects endogenous levels of Caspase-3 only when phosphorylated at serine 150.
  • Tested applications

    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-3.
    Database link: P42574

  • Positive control

    • Human lymph node tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab59425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.
WB 1/500 - 1/1000. Predicted molecular weight: 31 kDa.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificity

    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similarities

    Belongs to the peptidase C14A family.
  • Post-translational
    modifications

    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • A830040C14Rik antibody
    • Apopain antibody
    • CASP 3 antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • Caspase3 antibody
    • CC3 antibody
    • CPP 32 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • ICE3 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SCA1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    • Yama protein antibody
    see all

Images

  • ab59425, at a 1/50 dilution, staining paraffin embedded human lymph node tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.
  • All lanes : Anti-Caspase-3 (phospho S150) antibody (ab59425)

    Lane 1 : Jurkat cells, treated with Etoposide (25µM, 60mins)
    Lane 2 : Jurkat cells, treated with Etoposide (25µM, 60mins) with blocking phosphopeptide.

    Predicted band size: 31 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 23 kDa (possible isoform)

References

This product has been referenced in:

  • Li H  et al. Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis. Exp Ther Med 17:2310-2316 (2019). Read more (PubMed: 30867715) »
  • Li J  et al. Effect of long non-coding RNA Gas5 on proliferation, migration, invasion and apoptosis of colorectal cancer HT-29 cell line. Cancer Cell Int 18:4 (2018). Read more (PubMed: 29308053) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Loading amount
30 µg
Specification
HCT116
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 27 2012

Answer

Thank you for your inquiry.


If we know of our antibodies being used in any references/publications, we will list these under the "Specific References" section of our datasheet, which can be found under the shipping and pricing information online.


We know of one reference for ab112926: Okamoto K et al. DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation. Mol Cell Biol 25:9608-20 (2005). PubMed: 16227609


https://www.abcam.com/MDMX-phospho-S367-antibody-15-ab122926-references.html


We do not know of any publications for ab55334, ab59425, or ab28820. We would still guarantee these antibodies to work as stated on the datasheets in accordance with our Abpromise.


I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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