Key features and details
- Rabbit polyclonal to Caspase-3 (phospho S150)
- Suitable for: IHC-P, ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Caspase-3 (phospho S150) antibody
See all Caspase-3 primary antibodies
DescriptionRabbit polyclonal to Caspase-3 (phospho S150)
SpecificityCaspase-3 (Phospho-Ser150) antibody detects endogenous levels of Caspase-3 only when phosphorylated at serine 150.
Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Caspase-3.
Database link: P42574
- Human lymph node tissue
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab59425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site
Our Abpromise guarantee covers the use of ab59425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Predicted molecular weight: 31 kDa.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
Tissue specificityHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Sequence similaritiesBelongs to the peptidase C14A family.
modificationsCleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
- Information by UniProt
- A830040C14Rik antibody
- Apopain antibody
- CASP 3 antibody
ab59425, at a 1/50 dilution, staining paraffin embedded human lymph node tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.
All lanes : Anti-Caspase-3 (phospho S150) antibody (ab59425)
Lane 1 : Jurkat cells, treated with Etoposide (25µM, 60mins)
Lane 2 : Jurkat cells, treated with Etoposide (25µM, 60mins) with blocking phosphopeptide.
Predicted band size: 31 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa (possible isoform)
ab59425 has been referenced in 2 publications.
- Li H et al. Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis. Exp Ther Med 17:2310-2316 (2019). PubMed: 30867715
- Li J et al. Effect of long non-coding RNA Gas5 on proliferation, migration, invasion and apoptosis of colorectal cancer HT-29 cell line. Cancer Cell Int 18:4 (2018). PubMed: 29308053