Overview

  • Product name
    Anti-Caspase 4 antibody
    See all Caspase 4 primary antibodies
  • Description
    Rabbit polyclonal to Caspase 4
  • Host species
    Rabbit
  • Specificity
    ab22687 recognises proenzyme (43 kDa) and the large subunit (19 kDa) and small (10 kDa) subunits of mature enzyme. For the mature enzyme, reactivity is strongest with the large subunit. No cross reactivity with other human caspases tested (1 to 3, 5 to 10, 14).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human): Caspase 4

Properties

Applications

Our Abpromise guarantee covers the use of ab22687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/8000 - 1/16000. Detects bands of approximately 43, 19 and 10 kDa corresponding to the proenzyme, the large subunit and small subunits of mature enzyme, respectively (using Alkaline phosphatase-linked secondary antibody).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.
    • Tissue specificity
      Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.
    • Sequence similarities
      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Post-translational
      modifications
      The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.
    • Information by UniProt
    • Database links
      • Alternative names
        • Apoptotic cysteine protease Mih1/TX antibody
        • CASP-4 antibody
        • CASP4 antibody
        • CASP4_HUMAN antibody
        • Caspase 4 apoptosis related cysteine peptidase antibody
        • Caspase-4 subunit 2 antibody
        • Caspase4 antibody
        • ICE(rel)-II antibody
        • ICE(rel)II antibody
        • ICEREL II antibody
        • ICH2 antibody
        • Mih1/TX antibody
        • Protease ICH-2 antibody
        • Protease TX antibody
        • TX antibody
        see all

      References

      This product has been referenced in:
      • Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). Read more (PubMed: 28539405) »
      • Moorwood C & Barton ER Caspase-12 ablation preserves muscle function in the mdx mouse. Hum Mol Genet 23:5325-41 (2014). Read more (PubMed: 24879640) »

      See all 3 Publications for this product

      Publishing research using ab22687? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      3 reviews or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-Caspase 4 antibody

       Good
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (hepatocytes, Hep G2)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      15 µg
      Specification
      hepatocytes, Hep G2
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
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      Username

      Abcam user community

      Verified customer

      Submitted Jan 03 2018

      Phone call requesting infomration of the concentration of ab22687 as well as the recommended dilution for western blotting.

      Thank you for contacting us and sorry for the delay in getting back to you.

      I can now share with you that as discussed over the phone,this antibody is supplied as whole rabbit serum and theconcentration has therefore not been determined.Howev...

      Read More

      Last time you kindly advise our customer these suggestion below, our customer had change their condition as you recommended. 2) Load less sample protein i.e. ~20 ug/lane. 5) The recommended dilution in WB is 1/8000 - 1/16000 using Alkaline phosp...

      Read More

      Thank you for your reply. I am sorry that my suggestions did not improve the customer's results. Before I can consider offering you a free of charge replacement/refund, can you please provide a valid purchase order number for ab22687? The informat...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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