Anti-Caspase-4 antibody (ab22687)

Reviews (1)Q&A (2)References (4)

Key features and details

  • Rabbit polyclonal to Caspase-4
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Caspase-4 antibody
    See all Caspase-4 primary antibodies

  • Description

    Rabbit polyclonal to Caspase-4

  • Host species

    Rabbit

  • Specificity

    ab22687 recognises proenzyme (43 kDa) and the large subunit (19 kDa) and small (10 kDa) subunits of mature enzyme. For the mature enzyme, reactivity is strongest with the large subunit. No cross reactivity with other human caspases tested (1 to 3, 5 to 10, 14).

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human Caspase-4.

Properties

Applications

Our Abpromise guarantee covers the use of ab22687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/8000 - 1/16000. Detects bands of approximately 43, 19 and 10 kDa corresponding to the proenzyme, the large subunit and small subunits of mature enzyme, respectively (using Alkaline phosphatase-linked secondary antibody).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.

    • Tissue specificity

      Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.

    • Sequence similarities

      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.

    • Post-translational
      modifications

      The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.
    • Information by UniProt

    • Database links

      • Alternative names

        • Apoptotic cysteine protease Mih1/TX antibody
        • CASP-4 antibody
        • CASP4 antibody
        • CASP4_HUMAN antibody
        • Caspase 4 apoptosis related cysteine peptidase antibody
        • Caspase-4 subunit 2 antibody
        • Caspase4 antibody
        • ICE(rel)-II antibody
        • ICE(rel)II antibody
        • ICEREL II antibody
        • ICH2 antibody
        • Mih1/TX antibody
        • Protease ICH-2 antibody
        • Protease TX antibody
        • TX antibody
        see all

      References

      Publishing research using ab22687? Please let us know so that we can cite the reference in this datasheet.

      ab22687 has been referenced in 4 publications.

      • Chen N  et al. Cathepsin B regulates non-canonical NLRP3 inflammasome pathway by modulating activation of caspase-11 in Kupffer cells. Cell Prolif 51:e12487 (2018). PubMed: 30084208
      • Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). PubMed: 28539405
      • Moorwood C & Barton ER Caspase-12 ablation preserves muscle function in the mdx mouse. Hum Mol Genet 23:5325-41 (2014). PubMed: 24879640
      • Park GB  et al. Endoplasmic Reticulum Stress-Mediated Apoptosis of EBV-Transformed B Cells by Cross-Linking of CD70 Is Dependent upon Generation of Reactive Oxygen Species and Activation of p38 MAPK and JNK Pathway. J Immunol 185:7274-84 (2010). WB . PubMed: 21078900

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-Caspase 4 antibody

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (hepatocytes, Hep G2)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      15 µg
      Specification
      hepatocytes, Hep G2
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 03 2018

      Question

      Phone call requesting infomration of the concentration of ab22687 as well as the recommended dilution for western blotting.

      Read More
      Answer

      Thank you for contacting us and sorry for the delay in getting back to you.

      I can now share with you that as discussed over the phone,this antibody is supplied as whole rabbit serum and theconcentration has therefore not been determined.However, I can confirm that therecommended dilution for this antibody forWestern blottingis1:2000-1:4000. This has been amended fromthe original recommendation of 1/8000-1/16000.

      I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

      Read More

      Question

      Last time you kindly advise our customer these suggestion below, our customer had change their condition as you recommended. 2) Load less sample protein i.e. ~20 ug/lane. 5) The recommended dilution in WB is 1/8000 - 1/16000 using Alkaline phosphatase-linked secondary antibody. Since you used an HRP-conjugated secondary in ECL+, which is generally more sensitive than the AP detection, I suggest diluting ab22687 at ~1:20000-300000 and incubating at room temp for 1 hr or 4C overnight. protein loading 20 ug blocking:4?, overnight 2nd Ab: dilute 1/200, 000(jackson innunoresearch) We would be appreciated if you could provide them the credit note or a replacement vial. If there is any information you need, please do not hesitate to contact with me. Thanks for your help again.

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      Answer

      Thank you for your reply. I am sorry that my suggestions did not improve the customer's results. Before I can consider offering you a free of charge replacement/refund, can you please provide a valid purchase order number for ab22687? The information you provided (Abcam order or Purchase order number: Lot: _ 237881 c.) in previous e-mails is incorrect. I apologize for the inconvenience and I look forward to your reply.

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