Overview

  • Product name

  • Description

    Rabbit polyclonal to Caspase-4
  • Host species

    Rabbit
  • Specificity

    ab22687 recognises proenzyme (43 kDa) and the large subunit (19 kDa) and small (10 kDa) subunits of mature enzyme. For the mature enzyme, reactivity is strongest with the large subunit. No cross reactivity with other human caspases tested (1 to 3, 5 to 10, 14).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Caspase-4.

Properties

Applications

Our Abpromise guarantee covers the use of ab22687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/8000 - 1/16000. Detects bands of approximately 43, 19 and 10 kDa corresponding to the proenzyme, the large subunit and small subunits of mature enzyme, respectively (using Alkaline phosphatase-linked secondary antibody).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.
    • Tissue specificity

      Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.
    • Sequence similarities

      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Post-translational
      modifications

      The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apoptotic cysteine protease Mih1/TX antibody
      • CASP-4 antibody
      • CASP4 antibody
      • CASP4_HUMAN antibody
      • Caspase 4 apoptosis related cysteine peptidase antibody
      • Caspase-4 subunit 2 antibody
      • Caspase4 antibody
      • ICE(rel)-II antibody
      • ICE(rel)II antibody
      • ICEREL II antibody
      • ICH2 antibody
      • Mih1/TX antibody
      • Protease ICH-2 antibody
      • Protease TX antibody
      • TX antibody
      see all

    References

    This product has been referenced in:

    • Chen N  et al. Cathepsin B regulates non-canonical NLRP3 inflammasome pathway by modulating activation of caspase-11 in Kupffer cells. Cell Prolif 51:e12487 (2018). Read more (PubMed: 30084208) »
    • Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). Read more (PubMed: 28539405) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hepatocytes, Hep G2)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    hepatocytes, Hep G2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Jan 03 2018

    Answer

    Thank you for contacting us and sorry for the delay in getting back to you.

    I can now share with you that as discussed over the phone,this antibody is supplied as whole rabbit serum and theconcentration has therefore not been determined.However, I can confirm that therecommended dilution for this antibody forWestern blottingis1:2000-1:4000. This has been amended fromthe original recommendation of 1/8000-1/16000.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Answer

    Thank you for your reply. I am sorry that my suggestions did not improve the customer's results. Before I can consider offering you a free of charge replacement/refund, can you please provide a valid purchase order number for ab22687? The information you provided (Abcam order or Purchase order number: Lot: _ 237881 c.) in previous e-mails is incorrect. I apologize for the inconvenience and I look forward to your reply.

    Read More

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