Overview

  • Product name

    Caspase-4 Inhibitor Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Assay time

    1h 30m
  • Product overview

    Abcam's Caspase 4 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate LEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 4 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 4 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • Platform

    Microplate reader

Properties

Images

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using LEVD-AFC as caspase 4 substrate and following Caspase 4 Inhibitor Drug Detection Kit (ab102492) protocol. Lanes:

    1.- Background Control: no Caspase 4, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 4, +z-VAD-FMK

    3.- Positive Control: + Caspase 4, no z-VAD-FMK

    4.- Caspase 4 + 2.5µM z-VAD-FMK

    5.- Caspase 4 + 10µM z-VAD-FMK

    6.- Caspase 4 + 40µM z-VAD-FMK

Protocols

References

ab102492 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab102492.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up