Key features and details
- Rabbit polyclonal to Caspase-5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Caspase-5 antibody
See all Caspase-5 primary antibodies
DescriptionRabbit polyclonal to Caspase-5
Specificityab230840 detects endogenous levels of fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Caspase-5 aa 102-151. The exact sequence is proprietary.
Database link: P51878
- IHC-P: Human liver and small intestine tissues. WB: Extracts from HeLa and A549 cells, treated with etoposide.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add 1% BSA for extra stability. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
(PBS without Mg2+ and Ca2+).
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 50 kDa.|
FunctionMediator of programmed cell death (apoptosis).
Tissue specificityExpressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism.
- Information by UniProt
- Apoptosis related cysteine protease antibody
- CASP-5 antibody
- CASP5 antibody
All lanes : Anti-Caspase-5 antibody (ab230840) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), treated with etoposide 25 µM 24 hours cell lysate
Lane 2 : A549 (human lung carcinoma cell line), treated with etoposide 25 µM 24 hours cell lysate
Lane 3 : HeLa treated with etoposide 25 µM 24 hours cell lysate with synthesized peptide
Predicted band size: 50 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for Caspase 5 using ab230840 at 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human small intestine tissue stained for Caspase 5 using ab230840 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230840 has not yet been referenced specifically in any publications.