Overview

  • Product name

  • Description

    Rabbit polyclonal to Caspase-5
  • Host species

    Rabbit
  • Specificity

    ab230840 detects endogenous levels of fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Caspase-5 aa 102-151. The exact sequence is proprietary.
    Database link: P51878

  • Positive control

    • IHC-P: Human liver and small intestine tissues. WB: Extracts from HeLa and A549 cells, treated with etoposide.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add 1% BSA for extra stability. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol

    (PBS without Mg2+ and Ca2+).
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.

Target

  • Function

    Mediator of programmed cell death (apoptosis).
  • Tissue specificity

    Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications

    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis related cysteine protease antibody
    • CASP-5 antibody
    • CASP5 antibody
    • CASP5_HUMAN antibody
    • Caspase-5 subunit p10 antibody
    • ICE(rel)-III antibody
    • ICERELIII antibody
    • ICH 3 antibody
    • ICH 3 protease antibody
    • Protease ICH-3 antibody
    • Protease TY antibody
    • TY antibody
    • TY protease antibody
    see all

Images

  • All lanes : Anti-Caspase-5 antibody (ab230840) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), treated with etoposide 25 µM 24 hours cell lysate
    Lane 2 : A549 (human lung carcinoma cell line), treated with etoposide 25 µM 24 hours cell lysate
    Lane 3 : HeLa treated with etoposide 25 µM 24 hours cell lysate with synthesized peptide

    Predicted band size: 50 kDa

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Caspase 5 using ab230840 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human small intestine tissue stained for Caspase 5 using ab230840 at 1/50 dilution in immunohistochemical analysis.

References

ab230840 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230840.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up