Product nameAnti-Caspase-5 antibody
See all Caspase-5 primary antibodies
DescriptionRabbit polyclonal to Caspase-5
Specificityab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Caspase-5.
- Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMediator of programmed cell death (apoptosis).
Tissue specificityExpressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism.
- Information by UniProt
- Apoptosis related cysteine protease antibody
- CASP-5 antibody
- CASP5 antibody
All lanes : Anti-Caspase-5 antibody (ab69641) at 1/500 dilution
Lane 1 : HeLa cells treated with etoposide (25uM, 24hours)
Lane 2 :
A549 cells treated with etoposide (25uM, 24hours)
Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
Observed band size: 26,48 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible cleavage fragment)
ab69641 has not yet been referenced specifically in any publications.