Overview

  • Product name
    Anti-Caspase 5 antibody
    See all Caspase 5 primary antibodies
  • Description
    Rabbit polyclonal to Caspase 5
  • Host species
    Rabbit
  • Specificity
    ab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from human Caspase 5

  • Positive control
    • Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Mediator of programmed cell death (apoptosis).
    • Tissue specificity
      Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • Sequence similarities
      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Post-translational
      modifications
      The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • Database links
      • Alternative names
        • Apoptosis related cysteine protease antibody
        • CASP-5 antibody
        • CASP5 antibody
        • CASP5_HUMAN antibody
        • Caspase-5 subunit p10 antibody
        • ICE(rel)-III antibody
        • ICERELIII antibody
        • ICH 3 antibody
        • ICH 3 protease antibody
        • Protease ICH-3 antibody
        • Protease TY antibody
        • TY antibody
        • TY protease antibody
        see all

      Images

      • Western blot - Anti-Caspase 5 antibody (ab69641)
        Western blot - Anti-Caspase 5 antibody (ab69641)
        All lanes : Anti-Caspase 5 antibody (ab69641) at 1/500 dilution

        Lane 1 : HeLa cells treated with etoposide (25uM, 24hours)
        Lane 2 :
        A549 cells treated with etoposide (25uM, 24hours)
        Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 48 kDa
        Observed band size: 26,48 kDa (why is the actual band size different from the predicted?)
        Additional bands at: 26 kDa (possible cleavage fragment)

      References

      ab69641 has not yet been referenced specifically in any publications.

      Publishing research using ab69641? Please let us know so that we can cite the reference in this datasheet.

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