Anti-Caspase-5 antibody (ab69641)
Key features and details
- Rabbit polyclonal to Caspase-5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caspase-5 antibody
See all Caspase-5 primary antibodies -
Description
Rabbit polyclonal to Caspase-5 -
Host species
Rabbit -
Specificity
ab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Caspase-5.
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Positive control
- Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
| Notes |
|---|
WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Mediator of programmed cell death (apoptosis). -
Tissue specificity
Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism. - Information by UniProt
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Database links
- Entrez Gene: 838 Human
- Omim: 602665 Human
- SwissProt: P51878 Human
- Unigene: 213327 Human
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Alternative names
- Apoptosis related cysteine protease antibody
- CASP-5 antibody
- CASP5 antibody
see all
Images
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Western blot - Anti-Caspase-5 antibody (ab69641)All lanes : Anti-Caspase-5 antibody (ab69641) at 1/500 dilution
Lane 1 : HeLa cells treated with etoposide (25uM, 24hours)
Lane 2 :
A549 cells treated with etoposide (25uM, 24hours)
Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
Observed band size: 26,48 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible cleavage fragment)
Datasheets and documents
References (0)
ab69641 has not yet been referenced specifically in any publications.
