Key features and details
- Rabbit polyclonal to Caspase-5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Caspase-5 antibody
See all Caspase-5 primary antibodies
DescriptionRabbit polyclonal to Caspase-5
Specificityab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
Tested Applications & Species
Application Species WBHuman
Synthetic peptide corresponding to Human Caspase-5.
- Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMediator of programmed cell death (apoptosis).
Tissue specificityExpressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism.
- Information by UniProt
- Apoptosis related cysteine protease antibody
- CASP-5 antibody
- CASP5 antibody
All lanes : Anti-Caspase-5 antibody (ab69641) at 1/500 dilution
Lane 1 : HeLa cells treated with etoposide (25uM, 24hours)
Lane 2 :
A549 cells treated with etoposide (25uM, 24hours)
Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
Observed band size: 26,48 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible cleavage fragment)
ab69641 has not yet been referenced specifically in any publications.