Anti-Caspase-5 antibody (ab69641)

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Overview

  • Product name

    Anti-Caspase-5 antibody
    See all Caspase-5 primary antibodies

  • Description

    Rabbit polyclonal to Caspase-5

  • Host species

    Rabbit

  • Specificity

    ab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human Caspase-5.

  • Positive control

    • Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Mediator of programmed cell death (apoptosis).

    • Tissue specificity

      Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.

    • Sequence similarities

      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.

    • Post-translational
      modifications

      The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt

    • Database links

      • Alternative names

        • Apoptosis related cysteine protease antibody
        • CASP-5 antibody
        • CASP5 antibody
        • CASP5_HUMAN antibody
        • Caspase-5 subunit p10 antibody
        • ICE(rel)-III antibody
        • ICERELIII antibody
        • ICH 3 antibody
        • ICH 3 protease antibody
        • Protease ICH-3 antibody
        • Protease TY antibody
        • TY antibody
        • TY protease antibody
        see all

      Images

      • Western blot - Anti-Caspase-5 antibody (ab69641)
        Western blot - Anti-Caspase-5 antibody (ab69641)
        All lanes : Anti-Caspase-5 antibody (ab69641) at 1/500 dilution

        Lane 1 : HeLa cells treated with etoposide (25uM, 24hours)
        Lane 2 :
        A549 cells treated with etoposide (25uM, 24hours)
        Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 48 kDa
        Observed band size: 26,48 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 26 kDa (possible cleavage fragment)

      References

      ab69641 has not yet been referenced specifically in any publications.

      Publishing research using ab69641? Please let us know so that we can cite the reference in this datasheet.

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