Recombinant
RabMAb

Anti-Caspase 5 antibody [EP876Y] (ab40887)

Overview

  • Product name
    Anti-Caspase 5 antibody [EP876Y]
    See all Caspase 5 primary antibodies
  • Description
    Rabbit monoclonal [EP876Y] to Caspase 5
  • Host species
    Rabbit
  • Specificity
    Based on amino acids homology, this antibody will not recognize either p20 or p10 subunits of Caspase-5.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IP, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Caspase 5 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa cells, human breast carcinoma.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EP876Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Mediator of programmed cell death (apoptosis).
    • Tissue specificity
      Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • Sequence similarities
      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Post-translational
      modifications
      The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    Images

    • Anti-Caspase 5 antibody [EP876Y] (ab40887) at 1/10000 dilution + HeLa cell lysate at 10 µg

      Predicted band size: 47 kDa
      Observed band size: 47 kDa

    • Stainign of Caspase 5 on paraffin-embedded human breast carcinoma sections, using ab40557 at a 1:50 dilution.
    • ab40887 staining HeLa cells for Caspase 5 (dilution 1:100).

    References

    This product has been referenced in:
    • Manchado E  et al. Targeting Mitotic Exit Leads to Tumor Regression In Vivo: Modulation by Cdk1, Mastl, and the PP2A/B55a,d Phosphatase. Cancer Cell 18:641-54 (2010). WB ; Mouse . Read more (PubMed: 21156286) »
    • Williams DS  et al. Nonsense mediated decay resistant mutations are a source of expressed mutant proteins in colon cancer cell lines with microsatellite instability. PLoS One 5:e16012 (2010). Read more (PubMed: 21209843) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry. I have contacted the originators of these antibodies and here is the sequence information I obtained. ab25899: This immunogen sequence is proprietary, but we can run any alignments with the sequence that you require. ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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