Caspase 6 (active) Staining Kit - Green Fluorescence (ab219936)

Overview

  • Product name
    Caspase 6 (active) Staining Kit - Green Fluorescence
    See all Caspase 6 kits
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Caspase 6 (active) Staining Kit - Green Fluorescence (ab219936) is a sensitive fluorometric assay to measure caspase 6 activation in live cells. The assay uses FAM-VEID-FMK, which binds irreversibly to active caspase 6 in apoptotic cells. The fluorescent intensity of the FAM-VEID-FMK signal is proportional to the amount of active caspase 6 and can be easily detected at Ex/Em = 490/525 nm by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.

  • Notes

    Caspase activity assay kits are based on fluorescent inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Overexpression of caspase 6 promotes programmed cell death. It has been proven that caspase 6 has substrate selectivity for the peptide sequence Val-Glu-Ile-Asp (VEID). This kit uses FAM-VEID-FMK as a fluorescent indicator for caspase 6 activity. FAM-VEID-FMK irreversibly binds to activated caspase 6 in apoptotic cells.

  • Tested applications
    Suitable for: Flow Cyt, FMmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab219936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
FM Use at an assay dependent concentration.

Images

  • Detection of active Caspase 6 in Jurkat cells. Jurkat cells (3 x 105 cells/100 μL/well) were either untreated (control) or treated with 1 μM staurosporine for 3 hours. Cells were incubated with FAM-VEID-FMK for 1 hour at 37°C. The fluorescent signal was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader (Molecular Devices) using bottom read mode

  • Active caspase 6 staining in Jurkat cells. cells (3 x 105 cells/100 μL/well) were either untreated (A) or treated with 1 μM staurosporine for 3 hours (B). Cells were incubated with FAM-VEID-FMK for 1 hour at 37°C. Increase in fluorescent intensity was observed using a fluorescence microscope with a FITC channel.

Protocols

References

ab219936 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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