Caspase-6 Assay Kit (Colorimetric) (ab39709)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Caspase-6 Assay Kit (Colorimetric)
See all Caspase-6 kits -
Detection method
Colorimetric -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Assay time
2h 00m -
Product overview
Abcam's Caspase-6 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate VEID-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
Visit our FAQs page for tips and troubleshooting. -
Notes
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml Dilution Buffer 1 x 100ml DTT 1 x 400µl VEID-pNA 1 x 500µl -
Research areas
-
Relevance
Caspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei. -
Cellular localization
Cytoplasmic -
Alternative names
- Apoptotic Protease MCH2
- CASP6
- Caspase 6 apoptosis related cysteine protease
see all
Associated products
-
Related Products
Protocols
References
This product has been referenced in:
- Shabanzadeh AP et al. Targeting caspase-6 and caspase-8 to promote neuronal survival following ischemic stroke. Cell Death Dis 6:e1967 (2015). Read more (PubMed: 26539914) »
