Caspase 6 Assay Kit (Colorimetric) (ab39709)
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Overview
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Product nameCaspase 6 Assay Kit (Colorimetric)
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Detection methodColorimetric
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Sample typeTissue Extracts, Cell Lysate
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Assay typeEnzyme activity
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Assay time2h 00m
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Product overview
Abcam's Caspase 6 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate VEID-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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Notes
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
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PlatformMicroplate reader
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml Dilution Buffer 1 x 100ml DTT 1 x 400µl VEID-pNA 1 x 500µl -
Research areas
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RelevanceCaspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
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Cellular localizationCytoplasmic
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Alternative names
- Apoptotic Protease MCH2
- CASP6
- Caspase 6 apoptosis related cysteine protease
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Associated products
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Related Products
Protocols
References
ab39709 has not yet been referenced specifically in any publications.
