Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E180] to Caspase-6/CASP-6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Product nameAnti-Caspase-6/CASP-6 antibody [E180]
See all Caspase-6/CASP-6 primary antibodies
DescriptionRabbit monoclonal [E180] to Caspase-6/CASP-6
This antibody detects the pro form and the p11 subunit of human Caspase-6/CASP-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Caspase-6/CASP-6 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P55212
- WB: Jurkat cell lysate. ICC/IF: Hela cells. IHC-P: Human lung carcinoma tissue slides.
This product was previously labelled as Caspase-6.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Sequence similaritiesBelongs to the peptidase C14A family.
modificationsCleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
- Information by UniProt
- Apoptotic protease Mch-2 antibody
- Apoptotic protease MCH2 antibody
- CASP-6 antibody
All lanes : Anti-Caspase-6/CASP-6 antibody [E180] (ab32366) at 1/1000 dilution
Lane 1 : Untreated Jurkat cell lysate.
Lane 2 : Jurkat cell lysate treated with Camptothecin.
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Ab32366, at a 1:50 dilution, staining Caspase-6/CASP-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Ab32366, at a 1:100 dilution, staining Caspase-6/CASP-6 in Hela cells by Immunocytochemistry/Immunofluorescence.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32366 has been referenced in 1 publication.
- Coutinho-Camillo CM et al. Caspase expression in oral squamous cell carcinoma. Head Neck 33:1191-8 (2011). IHC ; Human . PubMed: 21755562