Product nameAnti-Caspase-6/CASP-6 antibody [EP1325Y]
See all Caspase-6/CASP-6 primary antibodies
DescriptionRabbit monoclonal [EP1325Y] to Caspase-6/CASP-6
ab52951 should recognize the pro-form of Caspase-6/CASP-6.
Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Caspase-6/CASP-6 aa 150-250. The exact sequence is proprietary.
Database link: P55212
- MCF-7 cell lysate, human breast carcinoma and HeLa cells.
A trial size is available to purchase for this antibody.
Previously labelled as Caspase-6.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval recommended
|ICC/IF||1/100 - 1/250.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Sequence similaritiesBelongs to the peptidase C14A family.
modificationsCleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
- Information by UniProt
- Apoptotic protease Mch-2 antibody
- Apoptotic protease MCH2 antibody
- CASP-6 antibody
Anti-Caspase-6/CASP-6 antibody [EP1325Y] (ab52951) at 1/20000 dilution + MCF-7 cell lysate at 10 µg
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
ab52951 at 1:100 dilution staining Caspase-6/CASP-6 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab52951 at 1:100 dilution staining Caspase-6/CASP-6 in HeLa cells by Immunofluorescence.
Overlay histogram showing Hela cells stained with ab52951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52951, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab52951 has not yet been referenced specifically in any publications.