Recombinant
RabMAb

Recombinant Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

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Overview

  • Product name

    Anti-Caspase-6/CASP-6 antibody [EPR18046]
    See all Caspase-6/CASP-6 primary antibodies

  • Description

    Rabbit monoclonal [EPR18046] to Caspase-6/CASP-6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Caspase-6/CASP-6 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P55212

  • Positive control

    • WB: Jurkat whole cell lysates untreated and treated with 1uM staurosporine for 4 hours; Mouse heart, mouse kidney, mouse spleen, rat brain and rat kidney lysates; RAW 264.7 whole cell lysate. IHC-P: Human colon, Human endometrium carcinoma, mouse kidney and rat kidney.

  • General notes

     

     

    Previously labelled as Caspase-6. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 33,11 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.

  • Sequence similarities

    Belongs to the peptidase C14A family.

  • Post-translational
    modifications

    Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

Images

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646) at 1/2000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 2 : Jurkat whole cell lysates treated with 1uM staurosporine for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 11,33 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646) at 1/2000 dilution

    Lane 1 : Mouse heart lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Mouse spleen lysates
    Lane 4 : Rat brain lysates
    Lane 5 : Rat kidney lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 1 minute


    The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646) at 1/2000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 11,33 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on the epithelial cells of Human colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

    Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on the cancer cells of endometrium carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on mouse kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on rat kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab185646 has not yet been referenced specifically in any publications.

Publishing research using ab185646? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-6 / CASP-6 antibody [EPR18046]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 1 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
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Mr. Carl Hobbs

Verified customer

Submitted Feb 13 2017

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