Overview

  • Product name

    Anti-Caspase-6/CASP-6 antibody [EPR18046]
    See all Caspase-6/CASP-6 primary antibodies
  • Description

    Rabbit monoclonal [EPR18046] to Caspase-6/CASP-6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Caspase-6/CASP-6 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P55212

  • Positive control

    • WB: Jurkat whole cell lysates untreated and treated with 1uM staurosporine for 4 hours; Mouse heart, mouse kidney, mouse spleen, rat brain and rat kidney lysates; RAW 264.7 whole cell lysate. IHC-P: Human colon, Human endometrium carcinoma, mouse kidney and rat kidney.
  • General notes

     

     

    Previously labelled as Caspase-6. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 33,11 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
  • Sequence similarities

    Belongs to the peptidase C14A family.
  • Post-translational
    modifications

    Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

Images

  • All lanes : Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646) at 1/2000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 2 : Jurkat whole cell lysates treated with 1uM staurosporine for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 11,33 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646) at 1/2000 dilution

    Lane 1 : Mouse heart lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Mouse spleen lysates
    Lane 4 : Rat brain lysates
    Lane 5 : Rat kidney lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 1 minute


    The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646) at 1/2000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 11,33 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on the epithelial cells of Human colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on the cancer cells of endometrium carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on mouse kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on rat kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab185646 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 1 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Feb 13 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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