Recombinant Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
![Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)](https://www.abcam.com/ps/products/108/ab108335/Images/ab108335-365995-anti-caspase-6-antibody-epr4405-western-blot-knockout-hap1-human-lysates.jpg "Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Caspase-6/CASP-6 antibody [EPR4405]
See all Caspase-6/CASP-6 primary antibodies -
Description
Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6 -
Host species
Rabbit -
Specificity
ab108335 detects both the full length and cleaved forms of Caspase-6/CASP-6.
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Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat cells and cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4405 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. -
Sequence similarities
Belongs to the peptidase C14A family. -
Post-translational
modificationsCleavages by caspase-3, caspase-8 or -10 generate the two active subunits. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 839 Human
- Entrez Gene: 12368 Mouse
- Entrez Gene: 83584 Rat
- Omim: 601532 Human
- SwissProt: P55212 Human
- SwissProt: O08738 Mouse
- SwissProt: O35397 Rat
- Unigene: 654616 Human
see all -
Alternative names
- Apoptotic protease Mch-2 antibody
- Apoptotic protease MCH2 antibody
- CASP-6 antibody
see all
Images
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Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CASP6 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 33 kDaLanes 1 - 2: Merged signal (red and green). Green - ab108335 observed at 37 kDa. Red - loading control, ab7291, observed at 50 kDa.
ab108335 was shown to specifically react with in wild-type HAP1 cells as signal was lost in CASP6 knockout cells. Wild-type and CASP6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab108335 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)](https://www.abcam.com/ps/products/108/ab108335/Images/ab108335-120917-anti-caspase-6-casp-6-antibody-epr4405-western-blot.jpg)
Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat + staurosporine cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
![Flow Cytometry (Intracellular) - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)](https://www.abcam.com/ps/products/108/ab108335/Images/ab108335-120916-anti-caspase-6-casp-6-antibody-epr4405-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)ab108335, at 1/10 dilution, staining Caspase-6/CASP-6 in permeabilized Jurkat cells by intracellular flow cytometry (shown in red). Rabbit IgG shown in green (negative).
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![Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)](https://www.abcam.com/ps/products/108/ab108335/Images/ab108335-4-benefits-of-recombinant-antibodies.png)
Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
Datasheets and documents
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Datasheet download
References (6)
ab108335 has been referenced in 6 publications.
- Zhu Y et al. Cardamonin inhibits cell proliferation by caspase-mediated cleavage of Raptor. Naunyn Schmiedebergs Arch Pharmacol 394:809-817 (2021). PubMed: 33043385
- Wang Y et al. Network Pharmacology-Based Strategy for the Investigation of the Anti-Obesity Effects of an Ethanolic Extract of Zanthoxylum bungeanum Maxim. Front Pharmacol 11:572387 (2020). PubMed: 33364948
- Mou Z et al. Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma. Front Genet 11:566918 (2020). PubMed: 33424916
- Volpin V et al. CAMK1D Triggers Immune Resistance of Human Tumor Cells Refractory to Anti-PD-L1 Treatment. Cancer Immunol Res 8:1163-1179 (2020). PubMed: 32665263
- Chen YJ et al. Nitrite Protects Neurons Against Hypoxic Damage Through S-nitrosylation of Caspase-6. Antioxid Redox Signal 31:109-126 (2019). PubMed: 30417658
- Ma J et al. Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res 26:713-27 (2016). PubMed: 27167569
