Recombinant Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)
![Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)](https://www.abcam.com/ps/products/247/ab247635/Images/ab247635-405872-anti-caspase-6-casp-6-antibody-epr4405-bsa-and-azide-free-western-blot-knockout-hap1-human-lysates.jpg "Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free
See all Caspase-6/CASP-6 primary antibodies -
Description
Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects both the full length and cleaved forms of Caspase-6/CASP-6.
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Tested applications
Suitable for: Flow Cyt, WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Caspase-6/CASP-6 aa 150-250. The exact sequence is proprietary.
Database link: P55212 -
General notes
Ab247635 is the carrier-free version of ab108335. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247635 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4405 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. -
Sequence similarities
Belongs to the peptidase C14A family. -
Post-translational
modificationsCleavages by caspase-3, caspase-8 or -10 generate the two active subunits. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 839 Human
- Entrez Gene: 12368 Mouse
- Entrez Gene: 83584 Rat
- Omim: 601532 Human
- SwissProt: P55212 Human
- SwissProt: O08738 Mouse
- SwissProt: O35397 Rat
- Unigene: 654616 Human
see all -
Alternative names
- Apoptotic protease Mch-2 antibody
- Apoptotic protease MCH2 antibody
- CASP-6 antibody
see all
Images
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Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CASP6 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 33 kDaThis data was developed using ab108335, the same antibody clone in a different buffer formulation.
Lanes 1 - 2: Merged signal (red and green). Green - ab108335 observed at 37 kDa. Red - loading control, ab7291, observed at 50 kDa.
ab108335 was shown to specifically react with in wild-type HAP1 cells as signal was lost in CASP6 knockout cells. Wild-type and CASP6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab108335 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)](https://www.abcam.com/ps/products/247/ab247635/Images/ab247635-402596-anti-caspase-6-casp-6-antibody-epr4405-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat + staurosporine cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDaThis data was developed using ab108335, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)](https://www.abcam.com/ps/products/247/ab247635/Images/ab247635-1-anti-caspase-6-casp-6-antibody-epr4405-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635) -
![Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)](https://www.abcam.com/ps/products/247/ab247635/Images/ab247635-4-benefits-of-recombinant-antibodies.png)
Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247635 has not yet been referenced specifically in any publications.
