Overview

  • Product name

    Anti-Caspase-6/CASP-6 antibody [EPR4405(2)]
    See all Caspase-6/CASP-6 primary antibodies
  • Description

    Rabbit monoclonal [EPR4405(2)] to Caspase-6/CASP-6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Caspase-6/CASP-6 aa 150-250. The exact sequence is proprietary.
    Database link: P55212

  • Positive control

    • Lysates of Jurkat cells treated with Camptothecin, Jurkat cells treated with Staurosporine, untreated Jurkat cells. Human lung carcinoma tissue.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Caspase-6. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
    • Sequence similarities

      Belongs to the peptidase C14A family.
    • Post-translational
      modifications

      Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apoptotic protease Mch-2 antibody
      • Apoptotic protease MCH2 antibody
      • CASP-6 antibody
      • CASP6 antibody
      • CASP6_HUMAN antibody
      • Caspase 6 antibody
      • Caspase 6 apoptosis related cysteine protease antibody
      • Caspase 6, apoptosis related cysteine peptidase antibody
      • Caspase-6 antibody
      • Caspase-6 subunit p11 antibody
      • Mch2 antibody
      • OTTHUMP00000162742 antibody
      • OTTHUMP00000162743 antibody
      see all

    Images

    • All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405(2)] (ab133259) at 1/1000 dilution

      Lane 1 : Lysate from Jurkat cells treated with Camptothecin
      Lanes 2 & 4 : Lysate from Jurkat cells
      Lane 3 : Lysate from Jurkat cells treated with Staurosporine

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 33 kDa

    • Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelling Caspase-6 / CASP-6 with ab133259 at 1:100.

    References

    ab133259 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133259.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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