Product nameAnti-Caspase-6/CASP-6 antibody [EPR4405(2)]
See all Caspase-6/CASP-6 primary antibodies
DescriptionRabbit monoclonal [EPR4405(2)] to Caspase-6/CASP-6
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human Caspase-6/CASP-6 aa 150-250. The exact sequence is proprietary.
Database link: P55212
- Lysates of Jurkat cells treated with Camptothecin, Jurkat cells treated with Staurosporine, untreated Jurkat cells. Human lung carcinoma tissue.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Caspase-6.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Sequence similaritiesBelongs to the peptidase C14A family.
modificationsCleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
- Information by UniProt
- Apoptotic protease Mch-2 antibody
- Apoptotic protease MCH2 antibody
- CASP-6 antibody
All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405(2)] (ab133259) at 1/1000 dilution
Lane 1 : Lysate from Jurkat cells treated with Camptothecin
Lanes 2 & 4 : Lysate from Jurkat cells
Lane 3 : Lysate from Jurkat cells treated with Staurosporine
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 33 kDa
Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelling Caspase-6 / CASP-6 with ab133259 at 1:100.
ab133259 has not yet been referenced specifically in any publications.