Anti-Caspase-7 antibody (ab25900)
Key features and details
- Rabbit polyclonal to Caspase-7
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Caspase-7 antibody
See all Caspase-7 primary antibodies -
Description
Rabbit polyclonal to Caspase-7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Caspase-7 aa 1-16 (C terminal).
Database link: P55210 -
Positive control
- Jurkat cell lysate, human skeletal muscle cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2 µg/ml.
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WB | (1) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
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Notes |
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IHC-P
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death. -
Tissue specificity
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. -
Sequence similarities
Belongs to the peptidase C14A family. -
Post-translational
modificationsCleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
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Alternative names
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
see all
Images
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Lane 1 : Anti-Caspase-7 antibody (ab25900) at 0.5 µg/ml
Lane 2 : Anti-Caspase-7 antibody (ab25900) at 1 µg/ml
Lane 3 : Anti-Caspase-7 antibody (ab25900) at 2 µg/ml
All lanes : Human skeletal
muscle cell lysate
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Immunohistochemical staining of human skeletal muscle (paraffin embedded sections) using ab25900 anti Caspase-7 at 2 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab25900 has been referenced in 8 publications.
- Meng L et al. Costunolide attenuates oxygen-glucose deprivation/reperfusion-induced mitochondrial-mediated apoptosis in PC12 cells. Mol Med Rep 23:N/A (2021). PubMed: 33786628
- Subhawa S et al. The Effects of Houttuynia cordata Thunb and Piper ribesioides Wall Extracts on Breast Carcinoma Cell Proliferation, Migration, Invasion and Apoptosis. Molecules 25:N/A (2020). PubMed: 32155880
- Zhao Y et al. Cortistatin binds to TNF-a receptors and protects against osteoarthritis. EBioMedicine 41:556-570 (2019). PubMed: 30826358
- Chen J et al. SM-164 enhances the antitumor activity of adriamycin in human U2-OS cells via downregulation of X-linked inhibitor of apoptosis protein. Mol Med Rep 19:5079-5086 (2019). PubMed: 31059038
- Yin Y et al. Expression of miRNA-214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis. Exp Ther Med 17:4657-4662 (2019). PubMed: 31086597
- Zhao Q et al. Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen-glucose deprivation/reoxygenation. Int J Mol Med 42:1190-1198 (2018). PubMed: 29786748
- Chen Q et al. USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway. Am J Physiol Cell Physiol 315:C863-C872 (2018). PubMed: 30281322
- Ma J et al. Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res 26:713-27 (2016). PubMed: 27167569