Key features and details
- Rabbit polyclonal to Caspase-7
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Caspase-7 antibody
See all Caspase-7 primary antibodies
DescriptionRabbit polyclonal to Caspase-7
SpecificityThe immunogen used for this product detects both caspase-7 precursor and small 105 aa C-terminal p12/12 kDa subunit in staurosporine (STS) treated cells.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
- This antibody gave a positive signal in the following lysates: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab92842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death.
Tissue specificityHighly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Sequence similaritiesBelongs to the peptidase C14A family.
modificationsCleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
- Information by UniProt
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Wild-type HAP1 treated with Staurosporin whole cell lysate (20 µg)
Lane 3: Caspase-7 knockout HAP1 whole cell lysate (20 µg)
Lane 4: Caspase-7 knockout HAP1 treated with Staurosporin whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: HeLa treated with Staurosporin whole cell lysate (20 µg)
Lanes 1 - 6: Merged signal (red and green). Green - ab92842 observed at 37, 12 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab92842 was shown to recognize Caspase-7 in wild-type cells as signal was lost at the expected MW in Caspase-7 knockout cells. Additional cross-reactive bands were observed in the wild-type and Caspase-7 knockout cells. Wild-type and Caspase-7 knockout samples were subjected to SDS-PAGE. Ab92842 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Caspase-7 antibody (ab92842) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
We hypothesize that the 15 kDa band represents the cleaved subunit (P11) of Caspase-7. Abcam welcomes customer feedback.
ICC/IF image of ab92845 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92842, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.
ab92842 has not yet been referenced specifically in any publications.