Overview

  • Product name

    Anti-Caspase-7 antibody - C-terminal
    See all Caspase-7 primary antibodies
  • Description

    Rabbit polyclonal to Caspase-7 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Caspase-7 (C terminal). The exact sequence is proprietary.
    Database link: P55210

  • Positive control

    • WB: HEK-293T, A431, HeLa, Jurkat and HepG2 whole cell extracts. IHC-P: Human hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • Tissue specificity

    Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • Sequence similarities

    Belongs to the peptidase C14A family.
  • Post-translational
    modifications

    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

Images

  • All lanes : Anti-Caspase-7 antibody - C-terminal (ab227959) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Caspase-7 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 34 kDa

  • Paraffin-embedded human hepatoma tissue stained for Caspase-7 using ab227959 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Caspase-7 antibody - C-terminal (ab227959) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : Caspase-7 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 34 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-Caspase-7 antibody - C-terminal (ab227959) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) treated with 10 µm Etoposide for 24 hours, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 34 kDa



    15% SDS-PAGE gel.

  • All lanes : Anti-Caspase-7 antibody - C-terminal (ab227959) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 34 kDa



    10% SDS-PAGE gel.

References

ab227959 has not yet been referenced specifically in any publications.

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