Anti-Caspase-7 antibody [E22] KO Tested (ab32522)

Product name

Anti-Caspase-7 antibody [E22]
See all Caspase-7 primary antibodies
Description

Rabbit monoclonal [E22] to Caspase-7
Host species

Rabbit
Specificity

The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members.
Tested applications

Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human Caspase-7 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P55210
Positive control
- WB: Jurkat & HeLa cell lysate. IHC-P: Human skin cancer tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes

A trial size is available to purchase for this antibody.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Clonality

Monoclonal
Clone number

E22
Isotype

IgG
Research areas

Alternative Versions
- Anti-Caspase-7 antibody [E22] (HRP) (ab206039)
Positive Controls
- Jurkat whole cell lysate (ab7899)

Our Abpromise guarantee covers the use of ab32522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		1/250.
WB		1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P		1/50 - 1/100.
ICC/IF		1/100.
IP		1/10.

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death.
Tissue specificity

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Sequence similarities

Belongs to the peptidase C14A family.
Post-translational
modifications

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P55210 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
Alternative names
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
- CASP7 antibody
- CASP7_HUMAN antibody
- Caspase 7 antibody
- Caspase 7 apoptosis related cysteine peptidase antibody
- Caspase-7 subunit p11 antibody
- Caspase7 antibody
- CMH 1 antibody
- CMH-1 antibody
- CMH1 antibody
- ICE LAP3 antibody
- ICE-LAP3 antibody
- ICE-like apoptotic protease 3 antibody
- LICE2 antibody
- MCH3 antibody
see all

Western blot - Anti-Caspase-7 antibody [E22] (ab32522)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Wild type HAP1 + Staurosporin whole cell lysate (20 µg)
Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
Lane 4: CASP7 + Staurosporin knockout HAP1 whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: HeLa + Staurosporin whole cell lysate (20 µg)

Lanes 1 - 6: Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab32522 was shown to specifically react with HAP1 + Staurosporin when HAP1 + Staurosporin knockout samples were used. Wild-type and HAP1 + Staurosporin knockout samples were subjected to SDS-PAGE. Ab32522 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Caspase-7 antibody [E22] (ab32522)

Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution + Jurkat cell lysate

Predicted band size: 34 kDa
Observed band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)

Immunohistochemical analysis of paraffin embedded human skin cancer tissue using ab32522 at 1:50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)

Immunofluorescent staining of HeLa cells using ab32522 at 1:100 dilution.
Flow Cytometry - Anti-Caspase-7 antibody [E22] (ab32522)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caspase-7 (red) with ab32522 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

Datasheet

This product has been referenced in:

Zhang B et al. Hypoxia-inducible factor-1 promotes cancer progression through activating AKT/Cyclin D1 signaling pathway in osteosarcoma. Biomed Pharmacother 105:1-9 (2018). WB . Read more (PubMed: 29807229) »
Chaudhary S et al. Overexpression of caspase 7 is ERa dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip). Oncogenesis 5:e219 (2016). WB, IF . Read more (PubMed: 27089142) »

See all 5 Publications for this product

Publishing research using ab32522? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunoprecipitation

Immuno-precipitation step

Protein A/G

Sample

Human Cell lysate - whole cell (MCF7)

Specification

MCF7

Total protein in input

100 µg

Abcam user community

Verified customer

Submitted Jul 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Loading amount

100 µg

Gel Running Conditions

Reduced Denaturing (8)

Sample

Human Cell lysate - whole cell (MCF7)

Specification

MCF7

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Jul 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Location selector

Your location is currently set to:

Partner with us

Anti-Caspase-7 antibody [E22] (ab32522)

Overview

Properties

Associated products

Applications

Target

Images

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Immunoprecipitation abreview for Anti-Caspase 7 antibody [E22]

Western blot abreview for Anti-Caspase-7 [E22] antibody