Anti-Caspase-7 antibody [E22] (ab32522)
Overview
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Product name
Anti-Caspase-7 antibody [E22]
See all Caspase-7 primary antibodies -
Description
Rabbit monoclonal [E22] to Caspase-7 -
Host species
Rabbit -
Specificity
The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members. -
Tested applications
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Caspase-7 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P55210 -
Positive control
- WB: Jurkat & HeLa whole cell lysate (ab150035). IHC-P: Human skin cancer tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
E22 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab32522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/250. | |
| WB | 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100. | |
| IP | 1/10. |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death. -
Tissue specificity
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. -
Sequence similarities
Belongs to the peptidase C14A family. -
Post-translational
modificationsCleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
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Alternative names
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
see all
Images
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![Western blot - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522-283568-anti-caspase-7-antibody-e22-western-blot.jpg)
Western blot - Anti-Caspase-7 antibody [E22] (ab32522)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Wild type HAP1 + Staurosporine ab120056 whole cell lysate (20 µg)
Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
Lane 4: CASP7 + Staurosporine knockout HAP1 whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: HeLa + Staurosporine whole cell lysate (20 µg)Lanes 1 - 6: Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab32522 was shown to specifically react with HAP1 + Staurosporine when HAP1 + Staurosporine knockout samples were used. Wild-type and HAP1 + Staurosporine knockout samples were subjected to SDS-PAGE. Ab32522 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522-45103-anti-caspase-7-antibody-e22-western-blot.gif)
Western blot - Anti-Caspase-7 antibody [E22] (ab32522)Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution + Jurkat cell lysate
Predicted band size: 34 kDa
Observed band size: 34 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522-45102-anti-caspase-7-antibody-e22-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)Immunohistochemical analysis of paraffin embedded human skin cancer tissue using ab32522 at 1:50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522-45101-anti-caspase-7-antibody-e22-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)Immunofluorescent staining of HeLa cells using ab32522 at 1:100 dilution.
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![Flow Cytometry - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522-280379-anti-caspase-7-antibody-e22-flow-cytometry.jpg)
Flow Cytometry - Anti-Caspase-7 antibody [E22] (ab32522)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caspase-7 (red) with ab32522 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab32522 has been referenced in 13 publications.
- Wang Y et al. A novel lncRNA MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis. Mol Cancer 18:28 (2019). PubMed: 30782188
- Wang Y et al. Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. Pathol Res Pract 215:1054-1060 (2019). PubMed: 30846414
- Zhang Y et al. Ailanthone up-regulates miR-449a to restrain acute myeloid leukemia cells growth, migration and invasion. Exp Mol Pathol 108:114-120 (2019). PubMed: 31002772
- Yuan Y & Zheng Z Geniposide protects PC-12 cells against oxygen and glucose deprivation-induced injury by up-regulation of long-noncoding RNA H19. Life Sci 216:176-182 (2019). PubMed: 30472296
- Sui H et al. Lidocaine inhibits growth, migration and invasion of gastric carcinoma cells by up-regulation of miR-145. BMC Cancer 19:233 (2019). PubMed: 30876463
