Anti-Caspase-7 antibody [E22] (ab32522)
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-Caspase-7 antibody [E22]
See all Caspase-7 primary antibodies -
DescriptionRabbit monoclonal [E22] to Caspase-7
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Host speciesRabbit
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SpecificityThe antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members.
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Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Caspase-7 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P55210 -
Positive control
- WB: Jurkat & HeLa whole cell lysate (ab150035). IHC-P: Human skin cancer tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
ClonalityMonoclonal
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Clone numberE22
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab32522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/250. | |
| WB | 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). | |
| IHC-P | 1/50 - 1/100. | |
| ICC/IF | 1/100. | |
| IP | 1/10. |
Target
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FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death. -
Tissue specificityHighly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
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Sequence similaritiesBelongs to the peptidase C14A family.
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Post-translational
modificationsCleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
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Alternative names
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
see all
Images
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![Western blot - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522-283568-ab32522SHKOWB.jpg)
Western blot - Anti-Caspase-7 antibody [E22] (ab32522)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Wild type HAP1 + Staurosporin whole cell lysate (20 µg)
Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
Lane 4: CASP7 + Staurosporin knockout HAP1 whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: HeLa + Staurosporin whole cell lysate (20 µg)Lanes 1 - 6: Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab32522 was shown to specifically react with HAP1 + Staurosporin when HAP1 + Staurosporin knockout samples were used. Wild-type and HAP1 + Staurosporin knockout samples were subjected to SDS-PAGE. Ab32522 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522wb.gif)
Western blot - Anti-Caspase-7 antibody [E22] (ab32522)Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution + Jurkat cell lysate
Predicted band size: 34 kDa
Observed band size: 34 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)Immunohistochemical analysis of paraffin embedded human skin cancer tissue using ab32522 at 1:50 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522if.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)Immunofluorescent staining of HeLa cells using ab32522 at 1:100 dilution.
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![Flow Cytometry - Anti-Caspase-7 antibody [E22] (ab32522)](https://www.abcam.com/ps/products/32/ab32522/Images/ab32522-4-ab32522FC1.jpg)
Flow Cytometry - Anti-Caspase-7 antibody [E22] (ab32522)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caspase-7 (red) with ab32522 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Datasheets and documents
References
This product has been referenced in:
- Yuan Y & Zheng Z Geniposide protects PC-12 cells against oxygen and glucose deprivation-induced injury by up-regulation of long-noncoding RNA H19. Life Sci 216:176-182 (2019). Read more (PubMed: 30472296) »
- Ding J et al. Overexpression of CXCL2 inhibits cell proliferation and promotes apoptosis in hepatocellular carcinoma. BMB Rep 51:630-635 (2018). Read more (PubMed: 30293547) »
