Overview

  • Product name
    Anti-Caspase-7 antibody [E22]
    See all Caspase-7 primary antibodies
  • Description
    Rabbit monoclonal [E22] to Caspase-7
  • Host species
    Rabbit
  • Specificity
    The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members.
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Caspase-7 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P55210

  • Positive control
    • WB: Jurkat & HeLa cell lysate. IHC-P: Human skin cancer tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/250.
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100.
IP 1/10.

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • Tissue specificity
    Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • Apoptotic protease Mch-3 antibody
      • Apoptotic protease MCH3 antibody
      • CASP-7 antibody
      • CASP7 antibody
      • CASP7_HUMAN antibody
      • Caspase 7 antibody
      • Caspase 7 apoptosis related cysteine peptidase antibody
      • Caspase-7 subunit p11 antibody
      • Caspase7 antibody
      • CMH 1 antibody
      • CMH-1 antibody
      • CMH1 antibody
      • ICE LAP3 antibody
      • ICE-LAP3 antibody
      • ICE-like apoptotic protease 3 antibody
      • LICE2 antibody
      • MCH3 antibody
      see all

    Images

    • Western blot - Anti-Caspase-7 antibody [E22] (ab32522)
      Western blot - Anti-Caspase-7 antibody [E22] (ab32522)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Wild type HAP1 + Staurosporin whole cell lysate (20 µg)
      Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
      Lane 4: CASP7 + Staurosporin knockout HAP1 whole cell lysate (20 µg)
      Lane 5: HeLa whole cell lysate (20 µg)
      Lane 6: HeLa + Staurosporin whole cell lysate (20 µg)

      Lanes 1 - 6: Merged signal (red and green). Green - ab32522 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab32522 was shown to specifically react with HAP1 + Staurosporin when HAP1 + Staurosporin knockout samples were used. Wild-type and HAP1 + Staurosporin knockout samples were subjected to SDS-PAGE. Ab32522 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Caspase-7 antibody [E22] (ab32522)
      Western blot - Anti-Caspase-7 antibody [E22] (ab32522)
      Anti-Caspase-7 antibody [E22] (ab32522) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size: 34 kDa
      Observed band size: 34 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [E22] (ab32522)

      Immunohistochemical analysis of paraffin embedded human skin cancer tissue using ab32522 at 1:50 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)
      Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [E22] (ab32522)

      Immunofluorescent staining of HeLa cells using ab32522 at 1:100 dilution.

    • Flow Cytometry - Anti-Caspase-7 antibody [E22] (ab32522)
      Flow Cytometry - Anti-Caspase-7 antibody [E22] (ab32522)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caspase-7 (red) with ab32522 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    References

    This product has been referenced in:
    • Zhang B  et al. Hypoxia-inducible factor-1 promotes cancer progression through activating AKT/Cyclin D1 signaling pathway in osteosarcoma. Biomed Pharmacother 105:1-9 (2018). WB . Read more (PubMed: 29807229) »
    • Chaudhary S  et al. Overexpression of caspase 7 is ERa dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip). Oncogenesis 5:e219 (2016). WB, IF . Read more (PubMed: 27089142) »

    See all 5 Publications for this product

    Publishing research using ab32522? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    Immunoprecipitation abreview for Anti-Caspase 7 antibody [E22]

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Human Cell lysate - whole cell (MCF7)
    Specification
    MCF7
    Total protein in input
    100 µg
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Jul 05 2013

    Western blot abreview for Anti-Caspase-7 [E22] antibody

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    100 µg
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Human Cell lysate - whole cell (MCF7)
    Specification
    MCF7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jul 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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