Overview

  • Product name

    Anti-Caspase-7 antibody [E22] (HRP)
    See all Caspase-7 primary antibodies
  • Description

    Rabbit monoclonal [E22] to Caspase-7 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity


    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human Caspase-7 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P55210

  • Positive control

    • WB: Jurkat whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • Tissue specificity

    Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • Sequence similarities

    Belongs to the peptidase C14A family.
  • Post-translational
    modifications

    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

Images

  • All lanes : Anti-Caspase-7 antibody [E22] (HRP) (ab206039) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CASP7 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    ab206039 was shown to specifically react with Caspase-7 in wild-type HAP1 cells as signal was lost in CASP7 knockout cells. Wild-type and CASP7 knockout samples were subjected to SDS-PAGE. Ab206039 was incubated overnight at 4°C at 1/5000 dilution. Blots were developed with ECL technique.

  • Anti-Caspase-7 antibody [E22] (HRP) (ab206039) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab206039 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab206039 has not yet been referenced specifically in any publications.

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