Recombinant
RabMAb

Recombinant Anti-Caspase-7 antibody [SP284] - C-terminal (ab226763)

Overview

  • Product name

    Anti-Caspase-7 antibody [SP284] - C-terminal
    See all Caspase-7 primary antibodies
  • Description

    Rabbit monoclonal [SP284] to Caspase-7 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Caspase-7 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P55210

  • Positive control

    • WB: Jurkat cell lysate. IHC-P: Human tonsil, endometrial adenocarcinoma, lung adenocarcinoma and breast ductal carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab226763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 28,32,34,38 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • Tissue specificity

    Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • Sequence similarities

    Belongs to the peptidase C14A family.
  • Post-translational
    modifications

    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

Images

  • Anti-Caspase-7 antibody [SP284] - C-terminal (ab226763) at 1/400 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Predicted band size: 28,32,34,38 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Caspase-7 using ab226763 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Caspase-7 using ab226763 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Caspase-7 using ab226763 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Caspase-7 using ab226763 at 1/100 dilution in immunohistochemical analysis.

References

ab226763 has not yet been referenced specifically in any publications.

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