Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP284] to Caspase-7 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-Caspase-7 antibody [SP284] - C-terminal
See all Caspase-7 primary antibodies
DescriptionRabbit monoclonal [SP284] to Caspase-7 - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Caspase-7 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P55210
- WB: Jurkat cell lysate. IHC-P: Human tonsil, endometrial adenocarcinoma, lung adenocarcinoma and breast ductal carcinoma tissues.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab226763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400. Predicted molecular weight: 28,32,34,38 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death.
Tissue specificityHighly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Sequence similaritiesBelongs to the peptidase C14A family.
modificationsCleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
- Information by UniProt
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
Anti-Caspase-7 antibody [SP284] - C-terminal (ab226763) at 1/400 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 28,32,34,38 kDa
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Caspase-7 using ab226763 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Caspase-7 using ab226763 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Caspase-7 using ab226763 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Caspase-7 using ab226763 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226763 has not yet been referenced specifically in any publications.