Overview

  • Product name
    Caspase-7 Immunoassay Activity Kit (Fluorometric)
  • Sample type
    Cell Lysate, Tissue Lysate
  • Assay type
    Quantitative
  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Abcam's Caspase 7 Immunoassay Kit (Fluorometric) provides an effective immunosorbent enzyme assay for the specific, quantitative detection of Caspase 7 activity in microtiter plates. The assay utilizes Caspase 7 polyclonal antibody to capture activated Caspase 7 from cell lysates. Substrate DEVD-AFC is then added that is cleaved proportionally to the amount of activated Caspase 7 in the cell lysate. The cleavage generates free AFC which is then analyzed fluorometrically (Ex./Em. = 400/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of Caspase 7. Other known caspases and non-specific proteases are not detected.

Properties

Protocols

References

This product has been referenced in:
  • Hassan I  et al. Restrained management of copper level enhances the antineoplastic activity of imatinib in vitro and in vivo. Sci Rep 8:1682 (2018). Read more (PubMed: 29374195) »

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