Abcam's Caspase 7 Immunoassay Kit (Fluorometric) provides an effective immunosorbent enzyme assay for the specific, quantitative detection of Caspase 7 activity in microtiter plates. The assay utilizes Caspase 7 polyclonal antibody to capture activated Caspase 7 from cell lysates. Substrate DEVD-AFC is then added that is cleaved proportionally to the amount of activated Caspase 7 in the cell lysate. The cleavage generates free AFC which is then analyzed fluorometrically (Ex./Em. = 400/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of Caspase 7. Other known caspases and non-specific proteases are not detected.
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Overexpression promotes programmed cell death.
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Belongs to the peptidase C14A family.
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.