Caspase-7 Inhibitor Assay Kit (ab102495)

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Overview

  • Product name

    Caspase-7 Inhibitor Assay Kit

  • Detection method

    Fluorescent

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Quantitative

  • Assay time

    1h 30m

  • Product overview

    Abcam's Caspase 7 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 7 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 7 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • Platform

    Microplate reader

Properties

Images

  • Caspase 7 Inhibitor Drug Detection Kit - ab102495
    Caspase 7 Inhibitor Drug Detection Kit - ab102495

    Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using DEVD-AFC as caspase substrate and following Caspase 7 Inhibitor Drug Detection Kit (ab102495) protocol. Lanes:

    1.- Background Control: no Caspase 7, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 7, +z-VAD-FMK

    3.- Positive Control: + Caspase 7, no z-VAD-FMK

    4.- Caspase 7 + 2.5µM z-VAD-FMK

    5.- Caspase 7 + 10µM z-VAD-FMK

    6.- Caspase 7 + 40µM z-VAD-FMK

Protocols

References

ab102495 has not yet been referenced specifically in any publications.

Publishing research using ab102495? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Hi dear

pls need ur kind help to send me data sheet regarding caspase 7 inhibitor drug detection kit, ab102495, as I dont know how I prepare my sample because it is not mentioned in the protocol.

Best regards

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Answer

Thank you again for contacting us.


I consulted the lab to make sure I can give you the information needed. The information in the protocol about sample preparation is very brief.

Under step one in the protocol for the kit you will find:

Prepare test samples in dH2O up to a final volume of 50 μL/well.

That means, that whatever sample you like to use (adherent cells or suspension cells) should be diluted in dH2O so that you will have the needed end concentration of cells in 50 μL you apply to every well.


The assay was tested useing approxamatly 1-5 x 10^6 cells. For the inhibitor, the same concentration range as mentioned for the control inhibitor (1 ul of 2 mM solution) should be used.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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