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    caspase-7-inhibitor-assay-kit-ab102495.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Inhibitors
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Caspase-7 Inhibitor Assay Kit (ab102495)

  • Datasheet
  • SDS
  • Protocol Booklet
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Caspase 7 Inhibitor Drug Detection Kit - ab102495

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Assay time: 1 hr 30 min
    • Sample type: Adherent cells, Suspension cells

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    Product image
    Human Caspase-7 ELISA Kit (ab274396)

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    Overview

    • Product name

      Caspase-7 Inhibitor Assay Kit
    • Detection method

      Fluorescent
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Quantitative
    • Assay time

      1h 30m
    • Product overview

      Abcam's Caspase 7 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 7 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 7 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
      Visit our FAQs page for tips and troubleshooting.

    • Notes

      Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 100 tests
      2X Reaction Buffer 1 x 10ml
      Active Caspase-7 1 x 100 units
      Caspase Inhibitor, Z-VAD-FMK 1 x 10µl
      Caspase Substrate DEVD-AFC 1 x 0.5ml
      DTT 1 x 100µl
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Caspases etc
      • Inhibitors
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Other
      • Kits/ Lysates/ Other
      • Kits
      • Apoptosis Kits
      • Other Apoptosis Kits
      • Kits/ Lysates/ Other
      • Kits
      • Apoptosis Kits
      • Caspase Assays Kits
      • Caspase 7 assay kits
      • Cancer
      • Cell Death
      • Apoptosis
      • Other
    • Relevance

      Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
    • Alternative names

      • CASP7 inhibitor

    Associated products

    • Related Products

      • Biotin-VAD-FMK, pan-caspase inhibitor (ab142032)
      • Biotin-DEVD-FMK, caspase-3 inhibitor (ab142033)
      • Boc-D-FMK, caspase inhibitor (ab142036)

    Images

    • Caspase 7 Inhibitor Drug Detection Kit - ab102495
      Caspase 7 Inhibitor Drug Detection Kit - ab102495

      Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using DEVD-AFC as caspase substrate and following Caspase 7 Inhibitor Drug Detection Kit (ab102495) protocol. Lanes:

      1.- Background Control: no Caspase 7, no z-VMAD-FMK

      2.- Positive Inhibition Control: no Caspase 7, +z-VAD-FMK

      3.- Positive Control: + Caspase 7, no z-VAD-FMK

      4.- Caspase 7 + 2.5µM z-VAD-FMK

      5.- Caspase 7 + 10µM z-VAD-FMK

      6.- Caspase 7 + 40µM z-VAD-FMK

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab102495? Please let us know so that we can cite the reference in this datasheet.

    ab102495 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Hi dear

    pls need ur kind help to send me data sheet regarding caspase 7 inhibitor drug detection kit, ab102495, as I dont know how I prepare my sample because it is not mentioned in the protocol.

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Oct 30 2012

    Answer

    Thank you again for contacting us.


    I consulted the lab to make sure I can give you the information needed. The information in the protocol about sample preparation is very brief.

    Under step one in the protocol for the kit you will find:

    Prepare test samples in dH2O up to a final volume of 50 μL/well.

    That means, that whatever sample you like to use (adherent cells or suspension cells) should be diluted in dH2O so that you will have the needed end concentration of cells in 50 μL you apply to every well.


    The assay was tested useing approxamatly 1-5 x 10^6 cells. For the inhibitor, the same concentration range as mentioned for the control inhibitor (1 ul of 2 mM solution) should be used.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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