For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    caspase-8-antibody-e7-ab32397.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Intracellular Caspases etc Caspases
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Caspase-8 antibody [E7] (ab32397)

  • Datasheet
Reviews (2) Submit a question References (23)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
  • Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
  • Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
  • Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
  • Anti-Caspase-8 antibody [E7] (ab32397)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E7] to Caspase-8
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

You may also be interested in

Knockout
Product image
Human CASP8 (Caspase-8) knockout HeLa cell lysate (ab256857)
Protein
Product image
Recombinant human Parathyroid Hormone protein (Active) (ab265858)
Protein
Product image
Recombinant human Caspase-8 protein (ab54117)

View more associated products

Overview

  • Product name

    Anti-Caspase-8 antibody [E7]
    See all Caspase-8 primary antibodies
  • Description

    Rabbit monoclonal [E7] to Caspase-8
  • Host species

    Rabbit
  • Specificity

    The antibody should recognize both pro-form (55kDa) and p18 cleaved-form of Caspase-8.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Caspase-8 aa 200-300 (N terminal). The exact sequence is proprietary.
    Database link: Q14790

  • Positive control

    • WB: Wild-type/Wild-type treated with Staurosporin HAP1, Jurkat, SH-SY5Y, IM-9, and HeLa (ab150035) whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E7
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Necroptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • HRP Anti-Caspase-8 antibody [E7] (ab194145)
    • Anti-Caspase-8 antibody [E7] - BSA and Azide free (ab232046)
  • Assay kits

    • Caspase-8 Assay Kit (Colorimetric) (ab39700)
    • Caspase-8 (active) FITC Staining Kit (ab65614)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CASP8 (Caspase-8) knockout HeLa cell line (ab264958)
  • KO cell lysates

    • Human CASP8 (Caspase-8) knockout HeLa cell lysate (ab256857)
  • KO cell pellets

    • Human CASP8 (Caspase-8) knockout HeLa cell pellet (ab278891)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant human Caspase-8 protein (ab54117)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

For unpurified use at 1/500.

Notes
WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

For unpurified use at 1/500.

Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • Tissue specificity

    Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • Involvement in disease

    Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Domain

    Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • Post-translational
    modifications

    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q14790 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 841 Human
    • Omim: 601763 Human
    • SwissProt: Q14790 Human
    • Unigene: 599762 Human
    • Alternative names

      • ALPS2B antibody
      • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
      • Apoptosis related cysteine peptidase antibody
      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch-5 antibody
      • Apoptotic protease Mch5 antibody
      • CAP 4 antibody
      • CAP4 antibody
      • CASP-8 antibody
      • CASP8 antibody
      • CASP8_HUMAN antibody
      • Caspase 8 antibody
      • Caspase 8 apoptosis related cysteine peptidase antibody
      • Caspase IIX antibody
      • Caspase-8 subunit p10 antibody
      • caspase8 antibody
      • CED 3 antibody
      • FADD Homologous ICE/CED3 Like Protease antibody
      • FADD Like ICE antibody
      • FADD-homologous ICE/CED-3-like protease antibody
      • FADD-like ICE antibody
      • FLICE antibody
      • FLJ17672 antibody
      • ICE-like apoptotic protease 5 antibody
      • MACH alpha 1/2/3 protein antibody
      • MACH antibody
      • MACH beta 1/2/3/4 protein antibody
      • MACH5 antibody
      • MCH 5 antibody
      • MCH5 antibody
      • MGC78473 antibody
      • MORT1 associated ced 3 homolog antibody
      • MORT1 associated CED3 homolog antibody
      • MORT1-associated CED-3 homolog antibody
      • OTTHUMP00000163717 antibody
      • OTTHUMP00000163720 antibody
      • OTTHUMP00000163724 antibody
      • OTTHUMP00000163725 antibody
      • OTTHUMP00000165062 antibody
      • OTTHUMP00000165063 antibody
      • OTTHUMP00000165064 antibody
      • OTTHUMP00000206552 antibody
      • OTTHUMP00000206582 antibody
      see all

    Images

    • Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
      Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
      All lanes : Anti-Caspase-8 antibody [E7] (ab32397) at 1/500 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : CASP8 knockout HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 55 kDa
      Observed band size: 55 kDa



      Lanes 1- 4: Merged signal (red and green). Green - ab32397 observed at 55 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab32397 was shown to react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type HeLa and CASP8 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
      Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
      All lanes : Anti-Caspase-8 antibody [E7] (ab32397) at 1/500 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : Wild-type HAP1 whole cell lysate treated with Staurosporin
      Lane 3 : Caspase-8 knockout HAP1 whole cell lysate
      Lane 4 : Caspase-8 knockout HAP1 whole cell lysate treated with Staurosporin

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 55 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab32397 observed at 55, 43/41 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab32397 was shown to specifically react with HAP1 + Staurosproin when HAP1 + Staurosproin knockout samples were used. Wild-type and HAP1 + Staurosproin knockout samples were subjected to SDS-PAGE. Ab32397 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
      Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
      All lanes : Anti-Caspase-8 antibody [E7] (ab32397) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : IM-9 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 55 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
      Western blot - Anti-Caspase-8 antibody [E7] (ab32397)
      Anti-Caspase-8 antibody [E7] (ab32397) at 1/500 dilution (unpurified) + HeLa cell lysate at 10 µg

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Anti-Caspase-8 antibody [E7] (ab32397)
      Anti-Caspase-8 antibody [E7] (ab32397)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (23)

    Publishing research using ab32397? Please let us know so that we can cite the reference in this datasheet.

    ab32397 has been referenced in 23 publications.

    • Zhang Y  et al. Long Non-coding RNA CASC15 Promotes Intrahepatic Cholangiocarcinoma Possibly through Inducing PRDX2/PI3K/AKT Axis. Cancer Res Treat 53:184-198 (2021). PubMed: 33017884
    • Zhao A  et al. lncRNA TUSC7 inhibits osteosarcoma progression through the miR-181a/RASSF6 axis. Int J Mol Med 47:583-594 (2021). PubMed: 33416181
    • Albahde MAH  et al. CBL0137 administration suppresses human hepatocellular carcinoma cells proliferation and induces apoptosis associated with multiple cell death related proteins. Neoplasma 67:547-556 (2020). PubMed: 32202904
    • Ou R  et al. HPV16 E6 oncoprotein-induced upregulation of lncRNA GABPB1-AS1 facilitates cervical cancer progression by regulating miR-519e-5p/Notch2 axis. FASEB J 34:13211-13223 (2020). PubMed: 32844486
    • Yao J  et al. Rapamycin inhibits proliferation and apoptosis of retinoblastoma cells through PI3K/AKT signaling pathway. Oncol Lett 19:2950-2956 (2020). PubMed: 32218850
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-2 of 2 Abreviews

    Western blot abreview for Anti-Caspase-8 antibody [E7]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (CT26 cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Tris-Bis gel)
    Loading amount
    40 µg
    Specification
    CT26 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2021

    Western blot abreview for Anti-Caspase-8 antibody [E7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (OV90 cells)
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Glycine Gel)
    Loading amount
    50000 cells
    Specification
    OV90 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.