Overview

  • Product name

    Caspase-8 Assay Kit (Fluorometric)
    See all Caspase-8 kits
  • Detection method

    Fluorescent
  • Sample type

    Tissue Extracts, Cell Lysate
  • Assay type

    Enzyme activity
  • Assay time

    2h 00m
  • Product overview

    Abcam's Caspase-8 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 8 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

    Other caspase and apoptosis assays

    Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.

  • Platform

    Microplate reader

Properties

Protocols

References

This product has been referenced in:

  • Buvall L  et al. Orellanine specifically targets renal clear cell carcinoma. Oncotarget 8:91085-91098 (2017). Functional Studies . Read more (PubMed: 29207627) »
  • Bailey LJ  et al. Augmented trophoblast cell death in preeclampsia can proceed via ceramide-mediated necroptosis. Cell Death Dis 8:e2590 (2017). Read more (PubMed: 28151467) »
See all 2 Publications for this product

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