• Product name

    Anti-Caspase-8 p10 antibody [C502S]
  • Description

    Mouse monoclonal [C502S] to Caspase-8 p10
  • Host species

  • Specificity

    This antibody recognizes the 10 kDa small subunit of Caspase 8. Does not detect full-length caspase 8.
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-8 p10 (N terminal).



Our Abpromise guarantee covers the use of ab2553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml.


  • Function

    Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • Tissue specificity

    Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • Involvement in disease

    Caspase-8 deficiency
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Domain

    Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • Post-translational

    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptotic cysteine protease antibody
    • Apoptotic protease Mch-5 antibody
    • CAP4 antibody
    • CASP-8 antibody
    • CASP8 antibody
    • CASP8_HUMAN antibody
    • Caspase 8 antibody
    • Caspase-8 subunit p10 antibody
    • FADD-homologous ICE/CED-3-like protease antibody
    • FADD-like ICE antibody
    • FLICE antibody
    • ICE-like apoptotic protease 5 antibody
    • MACH antibody
    • MORT1-associated CED-3 homolog antibody
    see all


This product has been referenced in:

  • Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). Read more (PubMed: 28539405) »
  • Helmke C  et al. Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Res 26:914-34 (2016). Read more (PubMed: 27325299) »
See all 2 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A


Thank you for your message. As it has not been tested yet with mouse tissue, we cannot provide any guarantees, but we would be very interested to hear how it works out for you if you do decide to try it. If you have any more questions, please do contact us again.

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Ab2553 has only been tested so far for cross-reactivity with human and tested in Western blotting and IP. I would not recommend this antibody to try with mouse samples in IHC - For immunohistochemistry, the primary antibody should be raised in a species as phylogenetically different as possible to the species that your sample was obtained. This is to avoid the secondary antibody cross-reacting with endogenous immunoglobulins in the sample, leading to erroneous results. For instance, a primary antibody used to detect a protein in a sample from a mouse should not be raised in mouse or rat. A primary antibody raised in rabbit would be a more appropriate choice.

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Thank you for your enquiry. This antibody recognizes only the active form.

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The information I have on file and can release is that the immunogen is a 12 a.a peptide corresponding to the N-terminus of the p10 subunit of human caspase-8. I cannot release the exact sequence, but if customer is working on caspases, he or she can easily obtain the sequence by checking the caspase-8 sequence at clevage site.

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The customer's sequence seems to be derived from mouse caspase-8. ab2553 utilizes the sequence derived from human. There are a few amino acids are different between human and mouse caspase-8.

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The immunogen is the synthetic peptide corresponding to residues mapping at the N-terminus of the p10 fragment of caspase-8. Gloria

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Yes, the antibody only detects p10 fragment of the cleaved caspase-8.

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