Overview

  • Product name

    Caspase 9 (active) FITC Staining Kit
    See all Caspase-9 kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Enzyme activity
  • Assay time

    2h 00m
  • Product overview

    Abcam's Caspase 9 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of caspases plays a central role in apoptosis.

    Other caspase and apoptosis assays

    Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    FITC-LEHD-FMK 1 x 100µl
    Wash Buffer 2 x 100ml
    Z-VAD-FMK 1 x 10µl
  • Research areas

  • Relevance

    Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.
  • Alternative names

    • APAF3
    • CASP9
    • Caspase 9
    • ICE LAP6
    • MCH6
    see all

Protocols

References

This product has been referenced in:

  • Davargaon RS  et al. Trolox prevents high glucose-induced apoptosis in rat myocardial H9c2 cells by regulating GLUT-4 and antioxidant defense mechanism. IUBMB Life N/A:N/A (2019). Read more (PubMed: 31359611) »
  • Ju C  et al. Neuroprotective effect of chondroitin sulfate on SH-SY5Y cells overexpressing wild-type or A53T mutant a-synuclein. Mol Med Rep 16:8721-8728 (2017). Read more (PubMed: 28990084) »
See all 4 Publications for this product

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