Key features and details
- Mouse monoclonal [2-22] to Caspase-9
- Suitable for: IHC-P, WB
- Reacts with: Human, Monkey
- Isotype: IgG1
Product nameAnti-Caspase-9 antibody [2-22]
See all Caspase-9 primary antibodies
DescriptionMouse monoclonal [2-22] to Caspase-9
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Monkey
Recombinant full length protein corresponding to Human Caspase-9 (Propeptide).
Database link: P55211
- HeLa cell lysate
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 50% Glycerol, 0.00017% PMSF, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab69541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
- Information by UniProt
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus, vessels tissue labelling Caspase-9 with ab69541 at 10µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Caspase-9 with ab69541 at 10µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling Caspase-9 with ab69541 at 10µg/ml.
All lanes : Anti-Caspase-9 antibody [2-22] (ab69541)
Lane 1 : HeLa cell lysate
Lane 2 : Induced HeLa cell lysate
Predicted band size: 46 kDa
ab69541 has not yet been referenced specifically in any publications.