Anti-Caspase-9 antibody (ab2013)

Reviews (2)Q&A (5)References (9)

Overview

  • Product name
    Anti-Caspase-9 antibody
    See all Caspase-9 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-9
  • Host species
    Rabbit
  • Specificity
    No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase-9.
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9 aa 41-56.
    Sequence:

    EDIQRAGSGSRRDQAR


    Database link: P55211
    (Peptide available as ab7885)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab2013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 46 kDa.Can be blocked with Human Caspase-9 peptide (ab7885).

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificity
    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stage
    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Images

  • Western blot - Anti-Caspase-9 antibody (ab2013)
    Western blot - Anti-Caspase-9 antibody (ab2013)
    Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution + HeLa whole cell lysate

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

References

This product has been referenced in:
  • Zhao Q  et al. Rhein protects against cerebral ischemic-/reperfusion-induced oxidative stress and apoptosis in rats. Int J Mol Med 41:2802-2812 (2018). Read more (PubMed: 29436613) »
  • Luan L & Liang Z Tanshinone IIA protects murine chondrogenic ATDC5 cells from lipopolysaccharide-induced inflammatory injury by down-regulating microRNA-203a. Biomed Pharmacother 103:628-636 (2018). WB . Read more (PubMed: 29679904) »
See all 9 Publications for this product

Publishing research using ab2013? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-Caspase-9 antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Pig Cell lysate - other (Lion, muscle tissue)
Gel Running Conditions
Reduced Denaturing (8-16%)
Loading amount
150 µg
Specification
Lion, muscle tissue
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Mayra González

Verified customer

Submitted Jul 14 2016

Western blot abreview for Anti-Caspase 9 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (stomach cancer)
Loading amount
25 µg
Specification
stomach cancer
Treatment
10uM WIN 55,212-2 for 24hrs
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 27 2013

Question

DESCRIPTION OF THE PROBLEM Does the casp9 antibody ab2013 detect your casp9 protein ab52203? If not can you recommend an antibody that will detect it so I can order the protein and ab together next week? I would like to perform a binding / inhibition experiment using these products if they are compatible.

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Answer

Thank you for your enquiry. Antibody ab2013 will recognize the recombinant CASP9 protein ab52203. Based on the units of ab52203 per vial received, you will receive about 62ug recombinant caspase 9 protein in a vial of ab52203. I hope this is helpful. Please contact me again if you have any further questions.

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Question

Customer has found that ab2013 does not recognise the cleaved form of the protein following the supplier providing the information that it does. Offered a replacement vial of ab25758 as a replacement in return for an Abreview.

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Answer

Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged for a free of charge replacement vial of ab25758 to be dispatched immediately (order number: 135138). I hope that this does not affect your choice of Abcam in the future.

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Question

Antibody MUST recognise the cleaved (and precursor) of Caspase 8 and 9 in WB

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Answer

Thank you for your email. I have performed a search of our catalogue and I can tell you that ab28142 and ab2013 are your best antibodies for the detection of cleaved and uncleaved caspase 8 and 9 by western blotting. The notes of each antibody are as follows: ab28142 detects ~55 and 57 kDa proteins, corresponding to the apparent molecular masses of the proform of caspase-8 alpha and beta on SDS-PAGE immunoblots. In apoptotic cells, this antibody also detects the processed forms (42/44 kDa, 31, 26, 18 and 14kDa). ab2013 detects both the activated (35 or 37kDa cleaved fragment depending on activator) and non-activated caspase-9 (45kDa). I would recommend that you have a look at the antibody's respective datasheets by clicking on the links below. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

has anybody tried caspase 9 for immunohistochemistry? is there any information on this?

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Answer

No, to our knowledge, this anitbody has only been tested in WB.

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Question

DOES THIS AB RECOGNIZE MOUSE CASPASE 9? CAN THIS AB DETECT ONLY THE 46 KDA PROTEIN? DOES IT CROSS REACT WITH THE ACTIVE CASPASE 9 (36 KDA)?

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Answer

This antibody was made with a peptide located at amino acids 41-56 of human caspase-9. The peptide is located in the cleaved and activated fragment (35 or 37 kDa). Therefore, it should recognize the precursor and activated form although we have not tested the activated form. Our Western Blot tests show a strong, single band at 46kDa. It is human, mouse, and rat reactive.

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