Anti-Caspase-9 antibody (ab2013)

Product name

Anti-Caspase-9 antibody
See all Caspase-9 primary antibodies
Description

Rabbit polyclonal to Caspase-9
Host species

Rabbit
Specificity

No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase-9.
Tested applications

Suitable for: IP, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide corresponding to Human Caspase-9 aa 41-56.
Sequence:
EDIQRAGSGSRRDQAR

Database link: P55211
(Peptide available as ab7885)

Run BLAST with Run BLAST with
Positive control
- HeLa whole cell lysate

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Caspase-9 Antibody is affinity chromatography purified via peptide column.
Clonality

Polyclonal
Isotype

IgG
Research areas

Assay kits
- Caspase 9 Assay Kit (Colorimetric) (ab65608)
- Caspase 9 (active) FITC Staining Kit (ab65615)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Immunizing Peptide (Blocking)
- Human Caspase-9 peptide (ab7885)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Our Abpromise guarantee covers the use of ab2013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		Use at an assay dependent concentration.
WB		1/1000 - 1/2000. Predicted molecular weight: 46 kDa.Can be blocked with Human Caspase-9 peptide (ab7885).

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificity

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similarities

Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stage

Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
Post-translational
modifications

Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Target information above from: UniProt accession P55211 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 842 Human
- Entrez Gene: 12371 Mouse
- Entrez Gene: 58918 Rat
- Omim: 602234 Human
- SwissProt: P55211 Human
- SwissProt: Q8C3Q9 Mouse
- Unigene: 329502 Human
- Unigene: 88829 Mouse
- Unigene: 32199 Rat
see all
Alternative names
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
- Apoptotic protease Mch-6 antibody
- Apoptotic protease-activating factor 3 antibody
- CASP-9 antibody
- CASP9 antibody
- CASP9_HUMAN antibody
- Caspase 9 apoptosis related cysteine peptidase antibody
- Caspase 9 Dominant Negative antibody
- Caspase 9c antibody
- Caspase-9 antibody
- Caspase-9 subunit p10 antibody
- ICE LAP6 antibody
- ICE like apoptotic protease 6 antibody
- ICE-LAP6 antibody
- ICE-like apoptotic protease 6 antibody
- MCH6 antibody
- PPP1R56 antibody
- protein phosphatase 1, regulatory subunit 56 antibody
- RNCASP9 antibody
see all

Western blot - Anti-Caspase-9 antibody (ab2013)

Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution + HeLa whole cell lysate

Predicted band size: 46 kDa
Observed band size: 46 kDa

Western blot protocols
Immunoprecipitation protocols

Datasheet

This product has been referenced in:

Zhao Q et al. Rhein protects against cerebral ischemic-/reperfusion-induced oxidative stress and apoptosis in rats. Int J Mol Med 41:2802-2812 (2018). Read more (PubMed: 29436613) »
Luan L & Liang Z Tanshinone IIA protects murine chondrogenic ATDC5 cells from lipopolysaccharide-induced inflammatory injury by down-regulating microRNA-203a. Biomed Pharmacother 103:628-636 (2018). WB . Read more (PubMed: 29679904) »

See all 10 Publications for this product

Publishing research using ab2013? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-7 of 7 Abreviews or Q&A

Application

Western blot

Sample

Pig Cell lysate - other (Lion, muscle tissue)

Gel Running Conditions

Reduced Denaturing (8-16%)

Loading amount

150 µg

Specification

Lion, muscle tissue

Blocking step

Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mayra González

Verified customer

Submitted Jul 14 2016

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (stomach cancer)

Loading amount

25 µg

Specification

stomach cancer

Treatment

10uM WIN 55,212-2 for 24hrs

Gel Running Conditions

Reduced Denaturing (10% SDS-PAGE)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 27 2013

Question

DESCRIPTION OF THE PROBLEM Does the casp9 antibody ab2013 detect your casp9 protein ab52203? If not can you recommend an antibody that will detect it so I can order the protein and ab together next week? I would like to perform a binding / inhibition experiment using these products if they are compatible.

Abcam community

Verified customer

Asked on Nov 21 2011

Answer

Thank you for your enquiry. Antibody ab2013 will recognize the recombinant CASP9 protein ab52203. Based on the units of ab52203 per vial received, you will receive about 62ug recombinant caspase 9 protein in a vial of ab52203. I hope this is helpful. Please contact me again if you have any further questions.

Abcam Scientific Support

Answered on Nov 21 2011

Question

Customer has found that ab2013 does not recognise the cleaved form of the protein following the supplier providing the information that it does. Offered a replacement vial of ab25758 as a replacement in return for an Abreview.

Abcam community

Verified customer

Asked on Mar 20 2006

Answer

Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged for a free of charge replacement vial of ab25758 to be dispatched immediately (order number: 135138). I hope that this does not affect your choice of Abcam in the future.

Abcam Scientific Support

Answered on Mar 20 2006

Question

Antibody MUST recognise the cleaved (and precursor) of Caspase 8 and 9 in WB

Abcam community

Verified customer

Asked on Feb 22 2006

Answer

Thank you for your email. I have performed a search of our catalogue and I can tell you that ab28142 and ab2013 are your best antibodies for the detection of cleaved and uncleaved caspase 8 and 9 by western blotting. The notes of each antibody are as follows: ab28142 detects ~55 and 57 kDa proteins, corresponding to the apparent molecular masses of the proform of caspase-8 alpha and beta on SDS-PAGE immunoblots. In apoptotic cells, this antibody also detects the processed forms (42/44 kDa, 31, 26, 18 and 14kDa). ab2013 detects both the activated (35 or 37kDa cleaved fragment depending on activator) and non-activated caspase-9 (45kDa). I would recommend that you have a look at the antibody's respective datasheets by clicking on the links below. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Feb 24 2006

Question

has anybody tried caspase 9 for immunohistochemistry? is there any information on this?

Abcam community

Verified customer

Asked on Mar 13 2002

Answer

No, to our knowledge, this anitbody has only been tested in WB.

Abcam Scientific Support

Answered on Mar 13 2002

Question

DOES THIS AB RECOGNIZE MOUSE CASPASE 9? CAN THIS AB DETECT ONLY THE 46 KDA PROTEIN? DOES IT CROSS REACT WITH THE ACTIVE CASPASE 9 (36 KDA)?

Abcam community

Verified customer

Asked on Dec 12 2000

Answer

This antibody was made with a peptide located at amino acids 41-56 of human caspase-9. The peptide is located in the cleaved and activated fragment (35 or 37 kDa). Therefore, it should recognize the precursor and activated form although we have not tested the activated form. Our Western Blot tests show a strong, single band at 46kDa. It is human, mouse, and rat reactive.

Abcam Scientific Support

Answered on Dec 14 2000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Location selector

Your location is currently set to:

Custom solutions & partnerships

Anti-Caspase-9 antibody (ab2013)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Western blot abreview for Anti-Caspase-9 antibody

Western blot abreview for Anti-Caspase 9 antibody