Anti-Caspase-9 antibody (ab2013)
Key features and details
- Rabbit polyclonal to Caspase-9
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Caspase-9 antibody
See all Caspase-9 primary antibodies -
Description
Rabbit polyclonal to Caspase-9 -
Host species
Rabbit -
Specificity
No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase-9. -
Tested Applications & Species
Application Species WB MouseRatHuman -
Immunogen
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Caspase-9 Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Mouse
Rat
Human
|
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/2000. Predicted molecular weight: 46 kDa.
|
Notes |
---|
WB
1/1000 - 1/2000. Predicted molecular weight: 46 kDa. |
Target
-
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
Tissue specificity
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Developmental stage
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. -
Post-translational
modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
-
Database links
- Entrez Gene: 842 Human
- Entrez Gene: 12371 Mouse
- Entrez Gene: 58918 Rat
- Omim: 602234 Human
- SwissProt: P55211 Human
- SwissProt: Q8C3Q9 Mouse
- Unigene: 329502 Human
- Unigene: 88829 Mouse
see all -
Alternative names
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
see all
Images
-
All lanes : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa -
Lane 1 : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml
Lane 2 : Anti-Caspase-9 antibody (ab2013) at 2 µg/ml
Lane 3 : Anti-Caspase-9 antibody (ab2013) at 4 µg/ml
All lanes : Human heart lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa -
Lane 1 : Anti-Caspase-9 antibody (ab2013) at 0.5 µg/ml
Lane 2 : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml
Lane 3 : Anti-Caspase-9 antibody (ab2013) at 2 µg/ml
All lanes : Mouse spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa -
All lanes : Anti-Caspase-9 antibody (ab2013) at 0.5 µg
All lanes : Rat spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 46 kDa -
Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution + HeLa whole cell lysate
Predicted band size: 46 kDa
Observed band size: 46 kDa
Datasheets and documents
References (28)
ab2013 has been referenced in 28 publications.
- Bao J et al. Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis. Aging (Albany NY) 12:5152-5167 (2020). PubMed: 32182210
- Yuan B et al. Role of Bcl-2 on drug resistance in breast cancer polyploidy-induced spindle poisons. Oncol Lett 19:1701-1710 (2020). PubMed: 32194662
- Ren DL et al. Oridonin induces apoptosis in HGC-27 cells by activating the JNK signaling pathway. Oncol Lett 19:255-260 (2020). PubMed: 31897137
- Shang J et al. Over-expression of JAZF1 promotes cardiac microvascular endothelial cell proliferation and angiogenesis via activation of the Akt signaling pathway in rats with myocardial ischemia-reperfusion. Cell Cycle 18:1619-1634 (2019). PubMed: 31177938
- Jiang F et al. Potential Roles of miR-374a-5p in Mediating Neuroprotective Effects and Related Molecular Mechanism. J Mol Neurosci 69:123-132 (2019). PubMed: 31127537