Anti-Caspase-9 antibody (ab2013)

Reviews (2)Q&A (5)References (28)
Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)

Key features and details

  • Rabbit polyclonal to Caspase-9
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Rat
Human
Application Abreviews Notes
WB (2)
1/1000 - 1/2000. Predicted molecular weight: 46 kDa.
Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 46 kDa.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

  • Tissue specificity

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.

  • Developmental stage

    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.

  • Post-translational
    modifications

    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Images

  • Western blot - Anti-Caspase-9 antibody (ab2013)
    Western blot - Anti-Caspase-9 antibody (ab2013)
    All lanes : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 46 kDa

  • Western blot - Anti-Caspase-9 antibody (ab2013)
    Western blot - Anti-Caspase-9 antibody (ab2013)
    Lane 1 : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml
    Lane 2 : Anti-Caspase-9 antibody (ab2013) at 2 µg/ml
    Lane 3 : Anti-Caspase-9 antibody (ab2013) at 4 µg/ml

    All lanes : Human heart lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 46 kDa

  • Western blot - Anti-Caspase-9 antibody (ab2013)
    Western blot - Anti-Caspase-9 antibody (ab2013)
    Lane 1 : Anti-Caspase-9 antibody (ab2013) at 0.5 µg/ml
    Lane 2 : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml
    Lane 3 : Anti-Caspase-9 antibody (ab2013) at 2 µg/ml

    All lanes : Mouse spleen lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 46 kDa

  • Western blot - Anti-Caspase-9 antibody (ab2013)
    Western blot - Anti-Caspase-9 antibody (ab2013)
    All lanes : Anti-Caspase-9 antibody (ab2013) at 0.5 µg

    All lanes : Rat spleen lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 46 kDa

  • Western blot - Anti-Caspase-9 antibody (ab2013)
    Western blot - Anti-Caspase-9 antibody (ab2013)
    Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution + HeLa whole cell lysate

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

References (28)

Publishing research using ab2013? Please let us know so that we can cite the reference in this datasheet.

ab2013 has been referenced in 28 publications.

  • Bao J  et al. Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis. Aging (Albany NY) 12:5152-5167 (2020). PubMed: 32182210
  • Yuan B  et al. Role of Bcl-2 on drug resistance in breast cancer polyploidy-induced spindle poisons. Oncol Lett 19:1701-1710 (2020). PubMed: 32194662
  • Ren DL  et al. Oridonin induces apoptosis in HGC-27 cells by activating the JNK signaling pathway. Oncol Lett 19:255-260 (2020). PubMed: 31897137
  • Shang J  et al. Over-expression of JAZF1 promotes cardiac microvascular endothelial cell proliferation and angiogenesis via activation of the Akt signaling pathway in rats with myocardial ischemia-reperfusion. Cell Cycle 18:1619-1634 (2019). PubMed: 31177938
  • Jiang F  et al. Potential Roles of miR-374a-5p in Mediating Neuroprotective Effects and Related Molecular Mechanism. J Mol Neurosci 69:123-132 (2019). PubMed: 31127537
View all Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-Caspase-9 antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Pig Cell lysate - other (Lion, muscle tissue)
Gel Running Conditions
Reduced Denaturing (8-16%)
Loading amount
150 µg
Specification
Lion, muscle tissue
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Mayra González

Verified customer

Submitted Jul 14 2016

Western blot abreview for Anti-Caspase 9 antibody

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (stomach cancer)
Loading amount
25 µg
Specification
stomach cancer
Treatment
10uM WIN 55,212-2 for 24hrs
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 27 2013

Question

DESCRIPTION OF THE PROBLEM Does the casp9 antibody ab2013 detect your casp9 protein ab52203? If not can you recommend an antibody that will detect it so I can order the protein and ab together next week? I would like to perform a binding / inhibition experiment using these products if they are compatible.

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Answer

Thank you for your enquiry. Antibody ab2013 will recognize the recombinant CASP9 protein ab52203. Based on the units of ab52203 per vial received, you will receive about 62ug recombinant caspase 9 protein in a vial of ab52203. I hope this is helpful. Please contact me again if you have any further questions.

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Question

Customer has found that ab2013 does not recognise the cleaved form of the protein following the supplier providing the information that it does. Offered a replacement vial of ab25758 as a replacement in return for an Abreview.

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Answer

Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged for a free of charge replacement vial of ab25758 to be dispatched immediately (order number: 135138). I hope that this does not affect your choice of Abcam in the future.

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Question

Antibody MUST recognise the cleaved (and precursor) of Caspase 8 and 9 in WB

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Answer

Thank you for your email. I have performed a search of our catalogue and I can tell you that ab28142 and ab2013 are your best antibodies for the detection of cleaved and uncleaved caspase 8 and 9 by western blotting. The notes of each antibody are as follows: ab28142 detects ~55 and 57 kDa proteins, corresponding to the apparent molecular masses of the proform of caspase-8 alpha and beta on SDS-PAGE immunoblots. In apoptotic cells, this antibody also detects the processed forms (42/44 kDa, 31, 26, 18 and 14kDa). ab2013 detects both the activated (35 or 37kDa cleaved fragment depending on activator) and non-activated caspase-9 (45kDa). I would recommend that you have a look at the antibody's respective datasheets by clicking on the links below. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

has anybody tried caspase 9 for immunohistochemistry? is there any information on this?

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Answer

No, to our knowledge, this anitbody has only been tested in WB.

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Question

DOES THIS AB RECOGNIZE MOUSE CASPASE 9? CAN THIS AB DETECT ONLY THE 46 KDA PROTEIN? DOES IT CROSS REACT WITH THE ACTIVE CASPASE 9 (36 KDA)?

Read More
Answer

This antibody was made with a peptide located at amino acids 41-56 of human caspase-9. The peptide is located in the cleaved and activated fragment (35 or 37 kDa). Therefore, it should recognize the precursor and activated form although we have not tested the activated form. Our Western Blot tests show a strong, single band at 46kDa. It is human, mouse, and rat reactive.

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