Overview

  • Product name

  • Description

    Rabbit polyclonal to Caspase-9
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase-9.
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9 aa 41-56.
    Sequence:

    EDIQRAGSGSRRDQAR


    Database link: P55211
    (Peptide available as ab7885)

  • Positive control

    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab2013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 46 kDa.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificity

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stage

    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications

    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links

  • Alternative names

    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Images

  • Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution + HeLa whole cell lysate

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

References

This product has been referenced in:

  • Chen Z  et al. Protective effects of lncRNA H19 silence against hypoxia-induced injury in PC-12 cells by regulating miR-28. Int J Biol Macromol 121:546-555 (2019). Read more (PubMed: 30312698) »
  • Chen Y  et al. Non-covalent proteasome inhibitor PI-1840 induces apoptosis and autophagy in osteosarcoma cells. Oncol Rep 41:2803-2817 (2019). Read more (PubMed: 30864717) »
See all 14 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-2 of 2 Abreviews

Application
Western blot
Sample
Pig Cell lysate - other (Lion, muscle tissue)
Gel Running Conditions
Reduced Denaturing (8-16%)
Loading amount
150 µg
Specification
Lion, muscle tissue
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mayra González

Verified customer

Submitted Jul 14 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (stomach cancer)
Loading amount
25 µg
Specification
stomach cancer
Treatment
10uM WIN 55,212-2 for 24hrs
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 27 2013

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