Anti-Caspase-9 antibody (ab25758)
Key features and details
- Rabbit polyclonal to Caspase-9
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Caspase-9 antibody
See all Caspase-9 primary antibodies -
Description
Rabbit polyclonal to Caspase-9 -
Host species
Rabbit -
Specificity
ab25758 detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9. The antibody does not detect any other caspases. -
Tested Applications & Species
Application Species WB Mouse -
Immunogen
Synthetic peptide corresponding to Mouse Caspase-9.
(Peptide available asab38268) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Mouse
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Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa). |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
Tissue specificity
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Developmental stage
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. -
Post-translational
modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
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Database links
- Entrez Gene: 12371 Mouse
- SwissProt: Q8C3Q9 Mouse
- Unigene: 88829 Mouse
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Alternative names
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
see all
Images
Datasheets and documents
References (39)
ab25758 has been referenced in 39 publications.
- Qiao W et al. miR-224-5p protects dental pulp stem cells from apoptosis by targeting Rac1. Exp Ther Med 19:9-18 (2020). PubMed: 31897093
- Chen FX et al. Inflammation-dependent downregulation of miR-532-3p mediates apoptotic signaling in human sarcopenia through targeting BAK1. Int J Biol Sci 16:1481-1494 (2020). PubMed: 32226296
- Yang L et al. Therapeutic efficacy of catalpol against apoptosis in cardiomyocytes derived from human induced pluripotent stem cells. AMB Express 10:56 (2020). PubMed: 32198560
- Fujii Y et al. Purification and Functional Characterization of the Effects on Cell Signaling of Mytilectin: A Novel ß-Trefoil Lectin from Marine Mussels. Methods Mol Biol 2132:201-213 (2020). PubMed: 32306329
- Qin QJ et al. Rhynchophylline ameliorates myocardial ischemia/reperfusion injury through the modulation of mitochondrial mechanisms to mediate myocardial apoptosis. Mol Med Rep 19:2581-2590 (2019). PubMed: 30720139