Anti-Caspase-9 antibody (ab25758)

Reviews (1)Q&A (3)References (39)
Western blot - Anti-Caspase-9 antibody (ab25758)

    Key features and details

    • Rabbit polyclonal to Caspase-9
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Overview

    • Product name

      Anti-Caspase-9 antibody
      See all Caspase-9 primary antibodies

    • Description

      Rabbit polyclonal to Caspase-9

    • Host species

      Rabbit

    • Specificity

      ab25758 detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9. The antibody does not detect any other caspases.

    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data

    • Immunogen

      Synthetic peptide corresponding to Mouse Caspase-9.
      (Peptide available as ab38268)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab25758 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).

    Target

    • Function

      Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
      Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

    • Tissue specificity

      Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

    • Sequence similarities

      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.

    • Developmental stage

      Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.

    • Post-translational
      modifications

      Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
    • Information by UniProt

    • Database links

      • Alternative names

        • APAF-3 antibody
        • APAF3 antibody
        • Apoptosis related cysteine peptidase antibody
        • Apoptotic protease Mch-6 antibody
        • Apoptotic protease-activating factor 3 antibody
        • CASP-9 antibody
        • CASP9 antibody
        • CASP9_HUMAN antibody
        • Caspase 9 apoptosis related cysteine peptidase antibody
        • Caspase 9 Dominant Negative antibody
        • Caspase 9c antibody
        • Caspase-9 antibody
        • Caspase-9 subunit p10 antibody
        • ICE LAP6 antibody
        • ICE like apoptotic protease 6 antibody
        • ICE-LAP6 antibody
        • ICE-like apoptotic protease 6 antibody
        • MCH6 antibody
        • PPP1R56 antibody
        • protein phosphatase 1, regulatory subunit 56 antibody
        • RNCASP9 antibody
        see all

      Images

      • Western blot - Anti-Caspase-9 antibody (ab25758)
        Western blot - Anti-Caspase-9 antibody (ab25758)
        Anti-Caspase-9 antibody (ab25758) + mouse intestine tissue lysate

        Predicted band size: 46 kDa



        The antibody detects 45 kDa procaspase 9, 35/37 kDa cleaved immediate fragment and the 17-25 kDa fully cleaved caspase 9.

      References (39)

      Publishing research using ab25758? Please let us know so that we can cite the reference in this datasheet.

      ab25758 has been referenced in 39 publications.

      • Qiao W  et al. miR-224-5p protects dental pulp stem cells from apoptosis by targeting Rac1. Exp Ther Med 19:9-18 (2020). PubMed: 31897093
      • Chen FX  et al. Inflammation-dependent downregulation of miR-532-3p mediates apoptotic signaling in human sarcopenia through targeting BAK1. Int J Biol Sci 16:1481-1494 (2020). PubMed: 32226296
      • Yang L  et al. Therapeutic efficacy of catalpol against apoptosis in cardiomyocytes derived from human induced pluripotent stem cells. AMB Express 10:56 (2020). PubMed: 32198560
      • Fujii Y  et al. Purification and Functional Characterization of the Effects on Cell Signaling of Mytilectin: A Novel ß-Trefoil Lectin from Marine Mussels. Methods Mol Biol 2132:201-213 (2020). PubMed: 32306329
      • Qin QJ  et al. Rhynchophylline ameliorates myocardial ischemia/reperfusion injury through the modulation of mitochondrial mechanisms to mediate myocardial apoptosis. Mol Med Rep 19:2581-2590 (2019). PubMed: 30720139
      View all Publications for this product

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Question

      1st I have to thank you all or your cooperation and Kindness special thanks to xxxxxxxxxx.

      I will order Anti-Caspase 9 antibody (ab25758)

      I am just wondering if there is any way to make sure that this antibody is going to work well with me before getting it !! .

      Read More
      Answer

      Thank you for contacting us.

      As this is an exceptional free of charge replacement, I will process the order. Could you please confirm that you would like a free of charge replacement with ab25758 and not ab133520 (rabbit monoclonal I recommended with a high specificity)?

      Unfortunately there is no way of telling how this antibody will work in your experiment apart from trying it. However, please be reassured that this antibody is also covered by our abpromise guarantee.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Western blot abreview for Anti-Caspase 9 antibody

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Human breast tumour)
      Specification
      Human breast tumour
      Treatment
      2mg/kg doxorubicin and 0.1mg/kg zoledronic acid
      Gel Running Conditions
      Reduced Non-Denaturing (Native) (10 % polyacrylamide gel)
      Blocking step
      (agent) for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
      Read More

      Dr. Penelope Ottewell

      Verified customer

      Submitted Jul 04 2008

      Question

      Hello, I'm considering purchasing the caspase 9 antibody ab25758. I would like to know if you have any data regarding cross-reactivity of this antibody with any other human caspases (full length or cleaved). Thank you.

      Read More
      Answer

      I can confirm that the antibody does not detect any other caspases. Please do not hesitate to contact us if you require further information,

      Read More

      Question

      Customer has found that ab2013 does not recognise the cleaved form of the protein following the supplier providing the information that it does. Offered a replacement vial of ab25758 as a replacement in return for an Abreview.

      Read More
      Answer

      Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged for a free of charge replacement vial of ab25758 to be dispatched immediately (order number: 135138). I hope that this does not affect your choice of Abcam in the future.

      Read More

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