Anti-Caspase-9 antibody (ab25758)

Reviews (1)Q&A (3)References (13)

Overview

  • Product name
    Anti-Caspase-9 antibody
    See all Caspase-9 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-9
  • Host species
    Rabbit
  • Specificity
    ab25758 detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9. The antibody does not detect any other caspases.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-9.
    (Peptide available as ab38268)

Properties

Applications

Our Abpromise guarantee covers the use of ab25758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).Can be blocked with Mouse Caspase-9 peptide (ab38268).

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificity
    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stage
    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Images

  • Western blot - Anti-Caspase-9 antibody (ab25758)
    Western blot - Anti-Caspase-9 antibody (ab25758)
    Anti-Caspase-9 antibody (ab25758) + mouse intestine tissue lysate

    Predicted band size: 46 kDa



    The antibody detects 45 kDa procaspase 9, 35/37 kDa cleaved immediate fragment and the 17-25 kDa fully cleaved caspase 9.

References

This product has been referenced in:
  • Huang N  et al. MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7. BMC Cell Biol 19:1 (2018). Read more (PubMed: 29433423) »
  • Wang J  et al. Curcumin inhibits the growth of liver cancer stem cells through the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway. Exp Ther Med 15:3650-3658 (2018). Read more (PubMed: 29545895) »
See all 13 Publications for this product

Publishing research using ab25758? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

1st I have to thank you all or your cooperation and Kindness special thanks to xxxxxxxxxx.

I will order Anti-Caspase 9 antibody (ab25758)

I am just wondering if there is any way to make sure that this antibody is going to work well with me before getting it !! .

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Answer

Thank you for contacting us.

As this is an exceptional free of charge replacement, I will process the order. Could you please confirm that you would like a free of charge replacement with ab25758 and not ab133520 (rabbit monoclonal I recommended with a high specificity)?

Unfortunately there is no way of telling how this antibody will work in your experiment apart from trying it. However, please be reassured that this antibody is also covered by our abpromise guarantee.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Western blot abreview for Anti-Caspase 9 antibody

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Human breast tumour)
Specification
Human breast tumour
Treatment
2mg/kg doxorubicin and 0.1mg/kg zoledronic acid
Gel Running Conditions
Reduced Non-Denaturing (Native) (10 % polyacrylamide gel)
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Dr. Penelope Ottewell

Verified customer

Submitted Jul 04 2008

Question

Hello, I'm considering purchasing the caspase 9 antibody ab25758. I would like to know if you have any data regarding cross-reactivity of this antibody with any other human caspases (full length or cleaved). Thank you.

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Answer

I can confirm that the antibody does not detect any other caspases. Please do not hesitate to contact us if you require further information,

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Question

Customer has found that ab2013 does not recognise the cleaved form of the protein following the supplier providing the information that it does. Offered a replacement vial of ab25758 as a replacement in return for an Abreview.

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Answer

Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged for a free of charge replacement vial of ab25758 to be dispatched immediately (order number: 135138). I hope that this does not affect your choice of Abcam in the future.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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