Anti-Caspase-9 antibody (ab25758)

Rabbit polyclonal Caspase-9 antibody. Validated in WB and tested in Mouse, Rat, Human, Xenopus laevis. Cited in 19 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name

  • Description

    Rabbit polyclonal to Caspase-9
  • Host species

    Rabbit
  • Specificity

    ab25758 detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9. The antibody does not detect any other caspases.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-9.
    (Peptide available as ab38268)

Properties

Applications

Our Abpromise guarantee covers the use of ab25758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificity

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stage

    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications

    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links

  • Alternative names

    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Images

  • Anti-Caspase-9 antibody (ab25758) + mouse intestine tissue lysate

    Predicted band size: 46 kDa



    The antibody detects 45 kDa procaspase 9, 35/37 kDa cleaved immediate fragment and the 17-25 kDa fully cleaved caspase 9.

References

This product has been referenced in:

  • Liu G  et al. Long non-coding RNA THRIL promotes LPS-induced inflammatory injury by down-regulating microRNA-125b in ATDC5 cells. Int Immunopharmacol 66:354-361 (2019). Read more (PubMed: 30521964) »
  • Zhang J  et al. Synergistic cytotoxicity of homoharringtonine and etoposide in acute myeloid leukemia cells involves disrupted antioxidant defense. Cancer Manag Res 11:1023-1032 (2019). Read more (PubMed: 30774430) »
See all 31 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Western blot
Sample
Human Tissue lysate - whole (Human breast tumour)
Specification
Human breast tumour
Treatment
2mg/kg doxorubicin and 0.1mg/kg zoledronic acid
Gel Running Conditions
Reduced Non-Denaturing (Native) (10 % polyacrylamide gel)
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Dr. Penelope Ottewell

Verified customer

Submitted Jul 04 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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