Overview

  • Product name
    Anti-Caspase-9 antibody
    See all Caspase-9 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-9
  • Host species
    Rabbit
  • Specificity
    ab52298 detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp 353.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-9.
    (Peptide available as ab171520)

  • Positive control
    • Human lung carcinoma tissue.
  • General notes
    ab52298 is cleaved at Asp353

Properties

Applications

Our Abpromise guarantee covers the use of ab52298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20520761
IHC-Fr Use at an assay dependent concentration. PubMed: 21765948
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.Can be blocked with Caspase-9 peptide (ab171520).

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificity
    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stage
    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody (ab52298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody (ab52298)
    ab52298 at 1/50 dilution staining Caspase 9 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • Western blot - Anti-Caspase-9 antibody (ab52298)
    Western blot - Anti-Caspase-9 antibody (ab52298)
    All lanes : Anti-Caspase-9 antibody (ab52298) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate with Blocked with synthesized peptide

    Predicted band size: 46 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 antibody (ab52298)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 antibody (ab52298)Image from Polo ML et al, PLoS One. 2010 May 26;5(5):e10786, Fig 5.
    ab52298 staining Caspase 9 - Cleaved Asp353 in mouse primary C4-HI cancer cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 10% formalin for 20 minutes at room temperature and incubated overnight at 4°C with the primary antibody. The corresponding secondary FITC-conjugated antibody was used at 1/100 dilution and incubated for 1 hour at room temperature. Nuclei were stained red with propidium iodide.
  • Immunohistochemistry (Frozen sections) - Anti-Caspase-9 antibody (ab52298)
    Immunohistochemistry (Frozen sections) - Anti-Caspase-9 antibody (ab52298)Image from Kaur J et al., PLoS One. 2011;6(7):e22181. Epub 2011 Jul 12. Fig S3.; doi:10.1371/journal.pone.0022181July 12, 2011, PLoS ONE 6(7): e22181.
    Immunohistochemical analysis of frozen retina tissue, taken from wild-type (top) or S334ter rhodopsin transgenic rats (bottom).

    Caspase 9 - Cleaved Asp353 was stained using ab52298 at 1/100 dilution. An AlexaFluor® 488-conjugated IgG was used as the secondary antibody.

References

This product has been referenced in:
  • Li X  et al. Tunicamycin inhibits progression of glioma cells through downregulation of the MEG-3-regulated wnt/ß-catenin signaling pathway. Oncol Lett 15:8470-8476 (2018). Read more (PubMed: 29805584) »
  • Wang J  et al. Enhancer of zeste homolog 2 blockade by RNA interference is implicated with inhibited proliferation, invasion and promoted apoptosis in endometrial carcinoma. Oncol Lett 15:9429-9435 (2018). Read more (PubMed: 29805666) »

See all 14 Publications for this product

Publishing research using ab52298? Please let us know so that we can cite the reference in this datasheet.

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