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  1. Link

    caspase-9-antibody-ab52298.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Anti-Caspase-9 antibody (ab52298)

  • Datasheet
Reviews (1) Submit a question References (58)

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Western blot - Anti-Caspase-9 antibody (ab52298)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody (ab52298)

Key features and details

  • Rabbit polyclonal to Caspase-9
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Caspase-9 antibody [EPR18107] (ab202068)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Caspase-9 antibody
    See all Caspase-9 primary antibodies
  • Description

    Rabbit polyclonal to Caspase-9
  • Host species

    Rabbit
  • Specificity

    ab52298 detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp 353.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-9.
    (Peptide available as ab171520)

  • General notes

    ab52298 is cleaved at Asp353

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab52298 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Cytochrome C
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Cytochrome C
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Neuroscience
    • Processes

Associated products

  • Assay kits

    • Caspase 9 Assay Kit (Colorimetric) (ab65608)
    • Caspase 9 (active) FITC Staining Kit (ab65615)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Caspase-9 peptide (ab171520)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Caspase-9 protein (Tagged) (ab198061)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Predicted molecular weight: 46 kDa.Can be blocked with Caspase-9 peptide (ab171520).
Notes
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Predicted molecular weight: 46 kDa.Can be blocked with Caspase-9 peptide (ab171520).

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificity

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stage

    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications

    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Target information above from: UniProt accession P55211 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 842 Human
    • Entrez Gene: 12371 Mouse
    • Omim: 602234 Human
    • SwissProt: P55211 Human
    • SwissProt: Q8C3Q9 Mouse
    • Unigene: 329502 Human
    • Alternative names

      • APAF-3 antibody
      • APAF3 antibody
      • Apoptosis related cysteine peptidase antibody
      • Apoptotic protease Mch-6 antibody
      • Apoptotic protease-activating factor 3 antibody
      • CASP-9 antibody
      • CASP9 antibody
      • CASP9_HUMAN antibody
      • Caspase 9 apoptosis related cysteine peptidase antibody
      • Caspase 9 Dominant Negative antibody
      • Caspase 9c antibody
      • Caspase-9 antibody
      • Caspase-9 subunit p10 antibody
      • ICE LAP6 antibody
      • ICE like apoptotic protease 6 antibody
      • ICE-LAP6 antibody
      • ICE-like apoptotic protease 6 antibody
      • MCH6 antibody
      • PPP1R56 antibody
      • protein phosphatase 1, regulatory subunit 56 antibody
      • RNCASP9 antibody
      see all

    Images

    • Western blot - Anti-Caspase-9 antibody (ab52298)
      Western blot - Anti-Caspase-9 antibody (ab52298)
      All lanes : Anti-Caspase-9 antibody (ab52298) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
      Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate with Blocked with synthesized peptide

      Predicted band size: 46 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody (ab52298)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody (ab52298)
      ab52298 at 1/50 dilution staining Caspase 9 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (58)

    Publishing research using ab52298? Please let us know so that we can cite the reference in this datasheet.

    ab52298 has been referenced in 58 publications.

    • Jia G  et al. Neural stem cell-conditioned medium ameliorates Aß25-35-induced damage in SH-SY5Y cells by protecting mitochondrial function. Bosn J Basic Med Sci 21:179-186 (2021). PubMed: 32156251
    • Liu Y  et al. Preclinical Evaluation of Safety, Pharmacokinetics, Efficacy, and Mechanism of Radioprotective Agent HL-003. Oxid Med Cell Longev 2021:6683836 (2021). PubMed: 33688393
    • Ouyang B  et al. miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-?B pathway. Oncol Rep 45:N/A (2021). PubMed: 33649824
    • Liang X & Ju J Matrine inhibits ovarian cancer cell viability and promotes apoptosis by regulating the ERK/JNK signaling pathway via p38MAPK. Oncol Rep 45:N/A (2021). PubMed: 33786627
    • Yang X  et al. YIV-906 potentiated anti-PD1 action against hepatocellular carcinoma by enhancing adaptive and innate immunity in the tumor microenvironment. Sci Rep 11:13482 (2021). PubMed: 34188068
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-9 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Fish Tissue sections (retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9,0
    Permeabilization
    No
    Specification
    retina
    Blocking step
    kit ab236466 as blocking agent for 10 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 11 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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