Anti-Caspase-9 antibody (ab52298)
- Datasheet
- References (16)
- Protocols
Overview
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Product nameAnti-Caspase-9 antibody
See all Caspase-9 primary antibodies -
DescriptionRabbit polyclonal to Caspase-9
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Host speciesRabbit
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Specificityab52298 detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp 353.
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Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, ELISA, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Mouse Caspase-9.
(Peptide available asab171520) -
Positive control
- Human lung carcinoma tissue.
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General notesab52298 is cleaved at Asp353
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab52298 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab52298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 20520761 | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 21765948 | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/20000. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 46 kDa.Can be blocked with Caspase-9 peptide (ab171520). |
Target
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FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
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Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain. -
Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
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Post-translational
modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
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Database links
- Entrez Gene: 842 Human
- Entrez Gene: 12371 Mouse
- Entrez Gene: 58918 Rat
- Omim: 602234 Human
- SwissProt: P55211 Human
- SwissProt: Q8C3Q9 Mouse
- Unigene: 329502 Human
- Unigene: 88829 Mouse
see all -
Alternative names
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody (ab52298)ab52298 at 1/50 dilution staining Caspase 9 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide. -
Western blot - Anti-Caspase-9 antibody (ab52298)All lanes : Anti-Caspase-9 antibody (ab52298) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate with Blocked with synthesized peptide
Predicted band size: 46 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 antibody (ab52298)Image from Polo ML et al, PLoS One. 2010 May 26;5(5):e10786, Fig 5.ab52298 staining Caspase 9 - Cleaved Asp353 in mouse primary C4-HI cancer cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 10% formalin for 20 minutes at room temperature and incubated overnight at 4°C with the primary antibody. The corresponding secondary FITC-conjugated antibody was used at 1/100 dilution and incubated for 1 hour at room temperature. Nuclei were stained red with propidium iodide. -
Immunohistochemistry (Frozen sections) - Anti-Caspase-9 antibody (ab52298)Image from Kaur J et al., PLoS One. 2011;6(7):e22181. Epub 2011 Jul 12. Fig S3.; doi:10.1371/journal.pone.0022181July 12, 2011, PLoS ONE 6(7): e22181.Immunohistochemical analysis of frozen retina tissue, taken from wild-type (top) or S334ter rhodopsin transgenic rats (bottom).
Caspase 9 - Cleaved Asp353 was stained using ab52298 at 1/100 dilution. An AlexaFluor® 488-conjugated IgG was used as the secondary antibody.
Datasheets and documents
References
This product has been referenced in:
- Li X et al. Tunicamycin inhibits progression of glioma cells through downregulation of the MEG-3-regulated wnt/ß-catenin signaling pathway. Oncol Lett 15:8470-8476 (2018). Read more (PubMed: 29805584) »
- Wang J et al. Enhancer of zeste homolog 2 blockade by RNA interference is implicated with inhibited proliferation, invasion and promoted apoptosis in endometrial carcinoma. Oncol Lett 15:9429-9435 (2018). Read more (PubMed: 29805666) »
