Anti-Caspase-9 antibody (ab52298)

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Key features and details

  • Rabbit polyclonal to Caspase-9
  • Suitable for: ICC/IF, IHC-Fr, IHC-P, ELISA, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Caspase-9 antibody
    See all Caspase-9 primary antibodies

  • Description

    Rabbit polyclonal to Caspase-9

  • Host species

    Rabbit

  • Specificity

    ab52298 detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp 353.

  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-P, ELISA, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-9.
    (Peptide available as ab171520)

  • Positive control

    • Human lung carcinoma tissue.

  • General notes

    ab52298 is cleaved at Asp353

Properties

Applications

Our Abpromise guarantee covers the use of ab52298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20520761
IHC-Fr Use at an assay dependent concentration. PubMed: 21765948
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.Can be blocked with Caspase-9 peptide (ab171520).

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

  • Tissue specificity

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.

  • Developmental stage

    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.

  • Post-translational
    modifications

    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

Images

  • Western blot - Anti-Caspase-9 antibody (ab52298)
    Western blot - Anti-Caspase-9 antibody (ab52298)
    All lanes : Anti-Caspase-9 antibody (ab52298) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate with Blocked with synthesized peptide

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody (ab52298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody (ab52298)
    ab52298 at 1/50 dilution staining Caspase 9 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 antibody (ab52298)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 antibody (ab52298)Image from Polo ML et al, PLoS One. 2010 May 26;5(5):e10786, Fig 5.
    ab52298 staining Caspase 9 - Cleaved Asp353 in mouse primary C4-HI cancer cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 10% formalin for 20 minutes at room temperature and incubated overnight at 4°C with the primary antibody. The corresponding secondary FITC-conjugated antibody was used at 1/100 dilution and incubated for 1 hour at room temperature. Nuclei were stained red with propidium iodide.
  • Immunohistochemistry (Frozen sections) - Anti-Caspase-9 antibody (ab52298)
    Immunohistochemistry (Frozen sections) - Anti-Caspase-9 antibody (ab52298)Image from Kaur J et al., PLoS One. 2011;6(7):e22181. Epub 2011 Jul 12. Fig S3.; doi:10.1371/journal.pone.0022181July 12, 2011, PLoS ONE 6(7): e22181.
    Immunohistochemical analysis of frozen retina tissue, taken from wild-type (top) or S334ter rhodopsin transgenic rats (bottom).

    Caspase 9 - Cleaved Asp353 was stained using ab52298 at 1/100 dilution. An AlexaFluor® 488-conjugated IgG was used as the secondary antibody.

References

Publishing research using ab52298? Please let us know so that we can cite the reference in this datasheet.

ab52298 has been referenced in 37 publications.

  • Zheng Y  et al. Desflurane Preconditioning Protects Against Renal Ischemia-Reperfusion Injury and Inhibits Inflammation and Oxidative Stress in Rats Through Regulating the Nrf2-Keap1-ARE Signaling Pathway. Drug Des Devel Ther 14:1351-1362 (2020). PubMed: 32308368
  • Hu X  et al. LncRNA Oprm1 overexpression attenuates myocardial ischemia/reperfusion injury by increasing endogenous hydrogen sulfide via Oprm1/miR-30b-5p/CSE axis. Life Sci 254:117699 (2020). PubMed: 32437793
  • Zhou J  et al. CircRNA-ENO1 promoted glycolysis and tumor progression in lung adenocarcinoma through upregulating its host gene ENO1. Cell Death Dis 10:885 (2019). PubMed: 31767835
  • Proboningrat A  et al. The potency of chitosan-based Pinus merkusii bark extract nanoparticles as anti-cancer on HeLa cell lines. Vet World 12:1616-1623 (2019). PubMed: 31849424
  • Abdallah AN  et al. Evaluation of treatment of experimentally induced canine model of multiple sclerosis using laser activated non-expanded adipose derived stem cells. Res Vet Sci 125:71-81 (2019). PubMed: 31152923
View all Publications for this product

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