For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    caspase-9-inhibitor-assay-kit-ab102497.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Intracellular Caspases etc Caspases
Share by email

Caspase-9 Inhibitor Assay Kit (ab102497)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)
  • Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)
  • Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)
  • Caspase 9 Inhibitor Drug Detection Kit

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Assay time: 1 hr 30 min
  • Sample type: Adherent cells, Suspension cells

You may also be interested in

ELISA
Product image
Human Caspase-9 ELISA Kit (ab119508)
Assay
Product image
Caspase-3 Assay Kit (Colorimetric) (ab39401)
Primary
Product image
Anti-LC3B antibody (ab63817)

View more associated products

Overview

  • Product name

    Caspase-9 Inhibitor Assay Kit
    See all Caspase-9 kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Assay time

    1h 30m
  • Product overview

    Abcam's Caspase 9 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate LEHD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 9 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 9 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K159 Caspase-9 Inhibitor Drug Screening Kit (Fluorometric). K159-100 is the same size as the 100 test size of ab102497.

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    2X Reaction Buffer 1 x 10ml
    Active Caspase-9 1 x 100 units
    Caspase Inhibitor, Z-VAD-FMK (2 mM) 1 x 10µl
    Caspase Substrate LEHD-AFC (1 mM) 1 x 0.5ml
    DTT (1 M) 1 x 100µl
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Inhibitors
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • Apoptosis Kits
    • Other Apoptosis Kits
    • Kits/ Lysates/ Other
    • Kits
    • Apoptosis Kits
    • Caspase Assays Kits
    • Caspase 9 assay kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Other
  • Relevance

    Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.
  • Alternative names

    • APAF3
    • CASP9
    • Caspase 9
    • ICE LAP6
    • MCH6
    see all

Associated products

  • Related Products

    • Z-LEHD-FMK, caspase-9 inhibitor (ab142026)
    • Q-LEHD-OPh, caspase-9 inhibitor (ab142039)

Images

  • Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)
    Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)

    Titration of the caspase 9 inhibitor Z-LEHD-FMK (ab142026) (duplicates; +/- SD).

  • Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)
    Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)

    Titration of the caspase inhibitors Z-VAD(OMe)-FMK (ab120487) and Q-LEHD-Oph (ab142039) (duplicates; +/- SD).

  • Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)
    Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)

    Titration of caspase inhibitor Z-VAD-FMK (ab102497) (duplicates; +/- SD).

  • Caspase 9 Inhibitor Drug Detection Kit
    Caspase 9 Inhibitor Drug Detection Kit

    Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using LEHD-AFC as caspase 9 substrate and following Caspase 9 Inhibitor Drug Detection Kit (ab102493) protocol. Lanes:

    1.- Background Control: no Caspase 9, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 9, +z-VAD-FMK

    3.- Positive Control: + Caspase 9, no z-VAD-FMK

    4.- Caspase 9 + 2.5µM z-VAD-FMK

    5.- Caspase 9 + 10µM z-VAD-FMK

    6.- Caspase 9 + 40µM z-VAD-FMK

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab102497? Please let us know so that we can cite the reference in this datasheet.

ab102497 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Hi,

I had another question about the Caspase 9 Inhibitor Drug Detection Kit (ab102497). I have run the assay and the RFUs for the positive inhibition are about 10x the RFUs for the background control. Is this expected? I have not tried higher concentrations of VAD-FMK; should the concentration of VAD-FMK specified in the protocol fully inhibit caspase 9 activity? Which control would you suggest using for normalization?

Thank you,

Read More

Abcam community

Verified customer

Asked on Oct 02 2012

Answer

Thank you very much for your email and for your patience while I've been in touch with the lab regarding your enquiry.

My contact at the lab has informed me that the background control is the fluorescence emitted by the substrate and the reaction buffer. These values have to be subtracted from all values. Your signals from the active caspase activity should be taken as 100% activity and used for normalization. The signals you then get from the use of our caspase inhibitor will give you an idea of how inhibition looks and what kind of signals it produces. This can be reported in terms of % inhibition. Then you can use your experimental inhibitor to looks at the % inhibition it produces.
I hope that this information will be useful, but if you have further questions or need anything else, please let me know and I'll be happy to help.

Read More

Abcam Scientific Support

Answered on Oct 02 2012

Question

The volumes in the protocol for ab102497 looks to be for a 96-well plate. Are there any reported problems with decreasing volumes for use in a 384-well plate (if this has been explored)? Also from the website it is not clear what the concentration of Z-VAD-fmk is used. What is the stock concentration of Z-VAD-fmk? Just to clarify the 1uL Z-VAD-fmk into 49uL H20 is used for a completely inhibited control?

Read More

Abcam community

Verified customer

Asked on Mar 15 2012

Answer

Thank you for your inquiry.

1) We have not looked at decreasing volumes for 384-well plates. You may be
able to use these, but it will depend on the amount of caspase in the samples
and hence signal strength can be impacted.

2) The Caspase Inhibitor, Z-VAD-FMK is 2 mM. I have since updated the online datasheet.

Hence 1 ul in 50 ul would result in 40 uM; which should be adequate for
recombinant Caspases. If you're looking at cell lystaes, you should probably
increase Z-VAD-FMK final to about 2-5x.
The updated component list will look like this, but it takes a few days to update:


Components 1 kit
2X Reaction Buffer 1 x 10ml
Active Caspase-9 1 x 100 units
Caspase Inhibitor, Z-VAD-FMK (2 mM) 1 x 10µl
Caspase Substrate LEHD-AFC (1 mM) 1 x 0.5ml
DTT (1 M) 1 x 100µl



I hope this information helps. Please contact us with any other questions.

Read More

Abcam Scientific Support

Answered on Mar 15 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.